Ermakov et al., "Metabolic pathway optimization using ribosome binding site
variants and combinatorial gene assembly," Applied Microbiology and Biotechnology, vol.
Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site
joined to the promoter.
Primers (200ug/ml) were designed flanking the c1 gene and contained a ribosome binding site
upstream of the c1 gene start codon (Figure 3).