TP53mutated breast cancer shows upregulation of PGD, TK, and ribose 5-phosphate
isomerase A .
Analysis of correlation matrix showed high correlation of BCAAs and cysteine, PPP metabolites (except ribose 5-phosphate
), and inosine and its metabolites (hypoxanthine and xanthine).
It generates the reducing equivalent NADPH for anabolism and ribose 5-phosphate
for nucleotide synthesis via the pentose phosphate pathway .
The detection of one of the metabolites, ribose 5-phosphate
, in the reaction mixtures is particularly noteworthy as its availability means that RNA precursors could in theory give rise to RNA molecules that encode information, catalyze chemical reactions and replicate.
We developed a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/ MS) method to simultaneously analyze the intracellular sugar phosphates dihydroxyacetone phosphate (DHAP), glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate
, ribulose 5-phosphate, xylulose 5-phosphate, fructose 6-phosphate, glucose 6-phosphate, and sedoheptulose 7-phosphate in blood spots.