B: first stage of germination, with one rhizoid
and a perpendicular first prothallial cell, 11 days.
Thallus delicate (2.5-3 mm high) attached to the substrate by multicellular rhizoids
unicellular, emerging proximally from pericentral cells, separated by the wall of the parent cell.
In both species, the basal cell second division produces a brownish rhizoid
that emerges through the laesura, and forms the first initial filament cell (Fig.
But, in Chara rhizoids
, the membrane-bound vesicles that are located close to the rhizoid
apex appear to serve this function [ILLUSTRATION FOR FIGURE 1 OMITTED].
cm= meristematic cell, r= rhizoid
, zm= meristematic zone.
The polarity-determination window is specified by a simple assay in which the spore position is changed by 180 [degrees] at various times after the initiation of germination by light, but before rhizoid
emergence, and then the subsequent direction of rhizoid
emergence and growth is recorded (Edwards and Roux, 1994).
On the agar, colonies were yellow-orange, flat, rather small and had irregular edges (rhizoid
) but some colonies were more rhizoid
than others and had different sizes and shapes, non-adherent to the agar (Figure 3), translucent, pale yellow due to flexirubin type pigments but the color was of different intensities on Anacker and Ordal agar as described by Bernardet and Grimont (1989).
Purity of isolated culture was confirmed by identification of white clear rhizoid
shaped colony and also through microscopic observation using gram staining method.
vittata showed that spores germinated after eight days and they gave rise to a few cell filaments, and a rhizoid
able to differentiate.
First division produces a rhizoid
and a prothallial cell which cut perpendicularly (Fig.
Microscopic examination of young, actively growing moulds was on the basis of structures bearing spores and on the spores themselves; presence or absence of septation, rhizoid
or other tissues.