Thin-section electron microscopy of CNS tissue of the mice had visible rhabdovirus
particles, and immunohistochemical testing detected rabies viral antigens.
Rhabdo means rod-shaped; viruses the rhabdovirus
family have a distinct bullet shape.
During a viral metagenomic survey, conducted as described previously (3), of fecal samples collected from 4 red foxes (Vulpes vulpes) that were found dead in Alava, Basque Country, Spain, we identified the complete coding sequence and the partial leader and trailer sequence of a novel rhabdovirus
, tentatively called red fox fecal rhabdovirus
(RFFRV; 15,541 nt, GenBank accession no.
Total DNA and RNA were extracted from tissue samples (brain, liver, lung, and heart) and subjected to reverse transcription PCR for rhabdovirus
and flavivirus (2).
We report genetic and phylogenetic characterization of a dolphin rhabdovirus
(DRV) and evaluated the seroprevalence of DRV-neutralizing antibodies by using serum samples from various marine mammals collected during a 10-year period (2003-2012).
The characterization of a novel rhabdovirus
in bats with a history of human contact raises questions for further research, including health effects on the virus' hosts, seroprevalence, possible transmission by insect vectors, and the extent of zoonotic exposure in humans.
A novel rhabdovirus
associated with acute hemorrhagic fever in central Africa.
9% identity to representative rhabdovirus
sequences that are available for this region of the genome) (data not shown).
This study, together with an earlier study of the portal of entry of a rhabdovirus
(infectious hematopoietic necrosis virus) in salmonids (30), suggests that the skin of teleost fish represents an efficient portal of entry for certain viruses.
Six isolates were found by CF test to be indistinguishable from Barur virus, a rhabdovirus
Deduced structural model for animal rhabdovirus
Of the other rhabdovirus
antigens and immune reagents used to characterize the three virus unknowns, some antigens were sucrose-acetone-extracted infected mouse brain, while others were medium from infected Vero cell cultures.