Un corps dilate, distordu sans etre revulsant
, ne possedant aucun referent reconnaissable.
The medium was then removed and 150 [micro]l DMEM containing FBS was added to the TGF-[beta]l revulsant
(final concentration adjusted to 6 ng/ml) with increasing stepwise concentrations of PR (50, 100, 200 nM), TGF-[beta]I specific inhibitor SB-431542 (100 nM) (Sigma, USA) and a blank control.
Besides chemical revulsants
, physical factors such as mechanical strain [8, 9], shear stress [10,11], and compressive stress [12, 13] also play important roles in the osteogenic differentiation of MSCs.