A concentration-related increase in the number of revertant
colonies per plate in at least one strain with or without metabolic activation system;
(11) That includes the observation of trace amounts of dystrophin expression in myofibers of patients with DMD, (23) as well as the prevalence of revertant
No precipitation was observed at test item concentrations up to 5 mg/plate in the precipitation test, and no dose-related decreases in revertant
colony numbers compared to the negative control or effects on background lawn development, with or without S9-mix, were observed at any test item concentration in tester strain TA100 in the preliminary cytotoxicity test (data not shown).
However, a drawback preventing a useful application of such strategies is represented by the revertant
nature of metabolically adapted LSC, which implies the risk of seriously damaging the HSC pool while trying to eradicate LSC.
Table 2: Mutagenic potential expressed by mean and standard deviation of revertant
colonies per plate and mutagenicity index in the absence of metabolic activation of the ethanolic extract of Cochlospermum regium leaves.
Also, a major reduction of the induced revertants
was obtained for TA97, TA98, and strains even in the absence of metabolic activation (Table 2).
Evaluation of nonsynonymous SNVs confirmed some outbreak viruses to be revertants
of the vaccine AHSV-1 strain toward the virulent parental type.
A more practical approach, i.e., a transient expression assay skipping the revertant
selection by P12/UV, (39) was also developed for further isolation of PEX cDNAs including nine others, PEX1, PEX3, PEX5, PEX6, PEX12, PEX13, PEX14, PEX19, and PEX26 (21), (34), (40)-(48) (Table 3; Fig.
Consumption of meat mutagens was estimated by multiplying the frequency of cooked meat intake from the cooking questionnaires with measured individual heterocyclic amine (ng/g meat) or MDM levels (revertant
colonies/g meat) and standard (medium) portion size from the CHARRED Database (NCI 2006).
The results showed that only positive control plates induced an increase in the number of revertant
colonies relative to the negative control, indicating mutagenic activity reaching a mutagenic ratio of higher than 2.0 (Table 7).
This confirmed Darwin's great predictive insight in the simplest forms of life, and it gave me the idea to see whether in the same way, Revertant
normal cells might arise from the descendants of a tumor cell, in advance and at random.
Klein-Szanto, "Secretion of gelatinases and tissue inhibitors of metalloproteinases by human lung cancer cell lines and revertant
cell lines: not an invariant correlation with metastasis," International Journal of Cancer, vol.