reversed phase chromatography

re·versed phase chro·ma·tog·ra·phy

a form of partitionary chromatography in which the stationary phase is less polar than the mobile phase.
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Hydrophilic interaction liquid chromatography (HILIC) is a rapidly growing alternative to reversed phase chromatography for the separation of hydrophilic compounds under conditions of high concentrations of organic solvents on hydrophilic supports.
illustrated that on column immuno-precipitation using an anti-sialyl lewis x IgM antibody in line with reversed phase chromatography had the ability to differentiate between a stage-2 ductal carcinoma plasma and NIST normal pooled plasma.
Sample preparation for immuno-MS workflows generally involves affinity enrichment, buffer exchange, proteolysis, de-salting, drying, re-suspending and reversed phase chromatography. These disjointed, user-dependent protocols generally lead to highly variable results and can take more than 24 hrs to complete.
Based on their finding, it was postulated that, in reversed phase chromatography, the cis isomers for 3-acyl and 4-acyl elute before their trans counterparts and the opposite was observed in the case for 5-acyls.
The first portion of the study examined the effect of mobile phase conditions on a reversed phase chromatography step.
* Decolorization of crude filtered vancomycin using a strong base anion exchange resin improved the purification of the vancomycin with subsequent reversed phase chromatography. The decolorization also diminished fouling of the reversed phase packing material, and allowed an effective single reversed phase step to achieve purity levels of 87% with an overall process yield of 65%.
Uracil, which under reversed phase chromatography is poorly retained and is used as a standard void volume marker, was retained by the FAA columns and eluted at 3.6 min under these conditions.
Can be used in both normal and reversed phase chromatography with either polar or non-polar mobile phases.
Based on reversed phase chromatography (RPC) or weak cation exchanqe (WCX), the beads can fractionate mixed protein and peptide samples quickly and easily for downstream analysis such as mass spectrometry.
The antisense molecules that are currently in pre-clinical and clinical trials are either purified by reversed phase chromatography (RPC) or a combination of RPC and IEX.
Based on reversed phase chromatography (RPC) or weak cation exchange (WCX), the beads can fractionate mixed protein and peptide samples quickly and easily for downstream analysis such as mass spectrometry.
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