The separation mechanism is based on partitioning in reversed phase
and ZIC ion ex-change resulting in a mixed mode for the ranitidine hydrochloride.
High resolution reversed phase
high performance liquid chromatography of peptides and proteins.
The same cannot be said of bonded reversed phase
columns with their different chemistries.
The primary source of protein retention on reversed phase
is hydrophobic interaction between hydrophobic parts of amino acid residues and alkyl chains in the stationary phase.
The first portion of the study examined the effect of mobile phase conditions on a reversed phase
19) from Roche Diagnostics, and the Jupiter[TM] Proteo column (C12 reversed phase
There are two main types of phase separation: (1) the normal phase HPLC which consists of a polar column material and relatively nonpolar solvents, and (2) reversed phase
HPLC which consists of a nonpolar column material and relatively polar solvents.
It offers LC media and columns for reversed phase
, ion exchange, hydrophobic interaction, gel filtration, and affinity separations and K-Prime 40 Biochromatography systems for process LC.
The supernatant was injected directly onto an HPLC consisting of a reversed phase
column (Prodigy C18, 250 x 4.
It can be employed with everything from hexane/isopropanol to methanol/water phases due to its intermediate polarity which is between that of the original silica gel and reversed phase
Fractions from reversed phase
high-performance liquid chromatography showed strong antimicrobial activity against L.