reverse transcriptase polymerase chain reaction


Also found in: Acronyms.

reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
Farlex Partner Medical Dictionary © Farlex 2012

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn)
A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Reverse transcriptase polymerase chain reaction diagnosed 26.1% of tuberculous effusion.
A group in England and Wales collected nose and throat swabs from 711 patients of all ages who presented with influenzalike illness, and found 408 (57%) to be negative for influenza and RSV Of those, 2.2% (9) were found to have HMPV by reverse transcriptase polymerase chain reaction (RT-PCR) technology (Emerg.
The level of enzyme activity was found to correlate exactly with the concentration of the enzyme, as measured by both Western blot and reverse transcriptase polymerase chain reaction tests.
Choanal and/or cloacal swabs were obtained and submitted to Virginia's Division of Consolidated Laboratory Services (Richmond, VA, USA) for analysis with real-time reverse transcriptase polymerase chain reaction (RT-PCR).
Laboratory diagnosis of SARS was obtained by reverse transcriptase polymerase chain reaction (RT-PCR) or serologic testing (4,5).
Tafra analyzed 38 false-negative biopsy specimens taken from patients using reverse transcriptase polymerase chain reaction for mammoglobin and detected tumor cells in 55% of the nodes.
[1] Nonstandard abbreviations: RT-PCR, reverse transcriptase-PCR; EGFR, epidermal growth factor receptor; and CURT-PCR, calibrated user-friendly reverse transcriptase polymerase chain reaction.
Comparison of Vero cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA assay, and VecYest antigen assay for detection of West Nile virus in field-collected mosquitos.
Reverse transcriptase polymerase chain reaction (RT-PCR) [3] provides highly sensitive detection of mRNA sequences and has been used widely for the study of gene expression in malignant and physiological states (5, 6).
Sequences were obtained from reverse transcriptase polymerase chain reaction (RT-PCR)--amplified portions of the nucleoprotein (positions 1,157-1,477), based on the reference strain M13215 (17).
Enhanced reverse transcriptase polymerase chain reaction for prostate specific antigen as an indicator of true pathologic stage in patients with prostate cancer.

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