The retroviral vector
backbone used in this study, pRUF-IRES-GFP, is a Murine Leukemia Virus-Based Vector which utilizes a MLV long terminal repeat (LTR).
Replication competent avian leukokosis virus long terminal repeat with a splice acceptor (RCAS) is a replication-competent retroviral vector
system that allows in ovo sustained misexpression of a gene of interest.
Genotyping analysis of the DNA extracted from the tail of chimera embryos revealed that 3 out of 10 embryos at E17.5 harbored Oct4-EGFP and Cre transgenes as well as sequences of retroviral vector
carrying Oct4, Sox2, and Klf4 genes (Figure 4(d)).
Klatzmann, "Replication-competent vectors and empty virus-like particles: new retroviral vector
designs for cancer gene therapy or vaccines," Molecular Therapy, vol.
Another inherited immune disorder, ADA deficiency has been successfully treated with a retroviral vector
that delivers a functional copy of the ADA gene.
pBABE, a retroviral vector
; hPPE, human proenkephalin; hBMSCs, human bone marrow stem cells; pBABE-hBMSCs, the pBABE-hBMSC group; hPPE-hBMSCs, the hPPE-hBMSC group.
The MazF retroviral vector
for this study is manufactured at the GMP compliant Center for Cell and Gene Therapy Facility of Takara Bio (Kusatsu, Shiga, Japan).
It is also possible to <<reprogram>> autologous T lymphocytes via transduction with a retroviral vector
that carries TCRs specific for defined tumor antigens.
Construction of recombinant retroviral vectors
and retroviral transduction: The recombinant Moloney murine leukaemia virus based retroviral vector
pLTKSN was constructed as described earlier (11).
Inhibition of other lentiviral and retroviral vector
coli, is stably delivered to CD4 T cells by retroviral vector
. We have demonstrated that human T cells transduced with MazF can significantly inhibit replication of HIV in vitro, while not affecting cell growth.