Data collected included age at presentation, gender, medical history, physical examination, blood and bone marrow investigations done as diagnostic workup for AML patients such as, haemoglobin levels, blood cell count myeloperoxidase (MPO) activity, periodic acid-Schiff (PAS) and
reticulin staining as well as the final diagnosis.
Histologically, PXA is characterized by marked cellular pleomorphism including spindle cells, mononuclear or multinucleated giant tumor cells with bizarre nuclei, prominent lipid droplets in cytoplasm, frequent perivascular lymphocytic infiltration, and eosinophilic granular bodies together with a dense
reticulin network.
These sections were stained using the
Reticulin Method, which enhances basement membranes, and resulted in a black hue of
reticulin fibers (modified from Puchtler & Waldrop, 1978; Vidal, 1988).
This was followed by tissue processing, paraffin embedding and sectioning, staining with haematoxylin and eosin (HandE) and
reticulin were done routinely.
2%) very well differentiated cases, which were
reticulin stained to determine the trabecular cord thickness [Figure 4].
There was no increase in blasts, mast cells, or fibrosis (CD34, tryptase, and
reticulin, resp.
Reticulin silver impregnation showed an epithelioid-like structure.
In parallel,
reticulin staining showed a dense pericellular deposition pattern in mesenchymal regions and was negative in GFAP-positive areas (Figure 4(d)).
In some cases histopathological diagnoses were confirmed using special stains such as: periodic acid-Schiff (PAS), with and without diastase pre-digestion, trichrome and
reticulin stains.
The Bone marrow pictures in CML without treatment showing hypercellularity due to excessive proliferation of the granulocytes with myelocytes predominantly and presence of blast cells from <10% to >20% in the bone marrow and peripheral blood according to the world health organization criteria that divide the CML in to chronic, accelerated phases and blast crisis, there is decreased or normal or increased megekayrioposis as well as moderate to marked
reticulin fibrosis with presence of small megakaryocyte containing hypolobulated nuclei, sea-blue histiocytes and gaucher cell and these changes are return to the normal state after treatment and the immunohisto-chemistry is used for differentiating the myeloblastic and lymphoblastic crisis of CML [7,8].
Specimens were stained with Masson's trichrome and Sweet's
reticulin stain for assessment of fibrosis and structural changes.
Reticulin antibody, arthritis, and coeliac disease in children.
