Another approach was adopted by Kurata et al to assess reticulated platelet
using RNA-binding dyes and flow cytometric analysers, as these reticulated platelets
are newly released from the bone marrow which can help in determining the aetiology of thrombocytopenia.
Staining of RNA remnants of immature platelet (reticulated platelet
) was performed by thiazole orange reagent.
The more immature the platelet, the more it fluoresces, thus allowing an accurate immature platelet count without requiring a separate reticulated platelet
test performed by traditional flow cytometry.
These reticulated platelet
data form the basis for further investigating the clinical utility of this assay in conditions of decreased and increased platelet production.
The reticulated platelet
assay can provide similar information, as the reticulated platelet
count tends to vary proportionately with marrow function, and very high counts can be seen in the setting of peripheral platelet destruction.
counts in patients undergoing autologous bone marrow transplantation: an aid in assessing marrow recovery.
First and foremost, because reticulated platelet
measurement is not part of the routine CBC, a new test must be ordered separately.
Role of reticulated platelets
and platelet size heterogeneity on platelet activity after dual antiplatelet therapy with aspirin and clopidogrel in patients with stable coronary artery disease.
Reference intervals of reticulated platelets
and other platelet parameters and their associations.
(RP) were identified by the thiazole orange method : 10 [micro]l of PRP was incubated with 390 [micro]l of thiazole orange (Retic-Count; BD Biosciences) or PBS as control and anti-CD41 at room temperature for 10 minutes, in the dark.
The significance of platelets with increased RNA content (reticulated platelets
): a measure of the rate of thrombopoiesis.