restriction site


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re·stric·tion site

a site in nucleic acid in which the bordering bases are of such a type as to leave them vulnerable to the cleaving action of an endonuclease.
Synonym(s): cleavage site

re·stric·tion site

(rĕ-strik'shŭn sīt)
A site in nucleic acid in which the bordering bases are of such a type as to leave them vulnerable to the cleaving action of an endonuclease.
Synonym(s): cleavage site.

Patient discussion about restriction site

Q. Is anyone restricted to have barley? what is the benefit of having barley and what is the best way to consume them? Is anyone restricted to have barley?

A. It grows in many parts of the world. As it is a whole grain it is good for health. It has soluble fiber and reduces blood cholesterol and glucose. It is low in fat content. No fixed way is there to eat barley as it’s used as soup thickener; it’s used in baked foods. Many breakfast foods include barley as baked breads. It is found to harm none.

Q. Is exercise recommended during pregnancy, if yes, are there any restrictions during pregnancy? I am in my 13 weeks of pregnancy. I always try to keep me fit and I do cycling every morning, swimming and yoga. These days I feel my body is changing and I am feeling more tiresome and nauseated. Is exercise recommended during pregnancy, if yes, are there any restrictions during pregnancy?

A. I exercised all through my pregnancies. I only gained a total of 10-12 pounds each time. I had easy deliveries because of the exercise. This has nothing to do with nausea. I had 9 months of nausea the first time around. Don't overdo the exercise, walking is the best exercise ever, and I climbed hills and stairs and walked several miles a day. My shortest delivery time was 5 minutes. I almost did not make it to the hospital. All my babies were healthy.

Q. What actions should i take in order to keep my self in a sharp and restricted fitness control?

A. I would try some body weight circuits 3 to 4 times a week.

More discussions about restriction site
References in periodicals archive ?
Locations of the restriction sites (black hash marks), bacterial transposon Tn7 sites (grey triangles), polyhedrin promoter (angled arrow corresponding to the sequence below), SINV-3 open reading frames (dark closed arrows), and approximate location of the area detected by the polyclonal antibody preparation (pAb) are shown.
The nature of the TRFLP analysis makes definitive identification of TRFs difficult, because numerous unrelated bacteria groups may share restriction sites, and fluorochrome dye labels and purine content can influence sizing errors during analysis (Schutte et al.
In the current pilot study on Filipino population the predominant genotypes showed the absence of BsmI restriction site in both the male controls and BPH patients (BB and Bb).
The vaccine strain was positive for an additional SmaI restriction site in ORF 62 ([SmaI.sup.+]) as opposed to all the circulating wild-type VZV strains analyzed, which were negative ([SmaI.sup.-]).
The degree of linkage disequilibrium, however, varies among different ethnic populations, thus leading to a loss of functional correlation between the restriction site and BMD.
The analysis of restriction site variation of polymerase chain reaction (PCR)-amplified mtDNA is an effective and relatively simple method for species identification of adult specimens of fish species.
Binary character matrices included only restriction sites (Hillis 1996) from those enzymes that yielded more than one haplotype from all individuals treated.
This result indicates that the polymorphic BamHI restriction site is outside of the rbcL region and thus the 8.6-kb fragment detected by Hultquist et al.
The PEPCK-luciferase (Luc) construct contains the region from -592 (NheI restriction site) to +87 (Bg/II restriction site) of the rat proximal PEPCK promoter ligated into the pGL3-basic vector (Promega, Madison, WI), as previously described (8).
Data presented in Cronin (1992) were shown to differentiate the Alaska, British Columbia, and Oregon black-tailed deer mtDNA haplotypes from the mule deer and western white-tailed deer haplotypes by 25 restriction site changes, whereas the greatest number of restriction site changes within the clade which included western white-tailed deer and mule deer was six restriction site changes.
Further investigation (Skuder et al., 1995) revealed that this marker is a mitochondrial polymorphism, resulting from the loss of a MspI restriction site (MspI-) at position 15,925 of the mitochondrial genome (Anderson et al., 1981).
We have conducted simultaneous and separate parsimony analyses of two data sets, one comprising a miscellaneous set of "structural characters," representing variation in morphology, anatomy, biochemistry, and structural sequences of the chloroplast genome, the second reflecting cpDNA restriction site variation.