Locations of the restriction sites
(black hash marks), bacterial transposon Tn7 sites (grey triangles), polyhedrin promoter (angled arrow corresponding to the sequence below), SINV-3 open reading frames (dark closed arrows), and approximate location of the area detected by the polyclonal antibody preparation (pAb) are shown.
The nature of the TRFLP analysis makes definitive identification of TRFs difficult, because numerous unrelated bacteria groups may share restriction sites
, and fluorochrome dye labels and purine content can influence sizing errors during analysis (Schutte et al.
In the current pilot study on Filipino population the predominant genotypes showed the absence of BsmI restriction site
in both the male controls and BPH patients (BB and Bb).
The vaccine strain was positive for an additional SmaI restriction site
in ORF 62 ([SmaI.sup.+]) as opposed to all the circulating wild-type VZV strains analyzed, which were negative ([SmaI.sup.-]).
The degree of linkage disequilibrium, however, varies among different ethnic populations, thus leading to a loss of functional correlation between the restriction site
The analysis of restriction site
variation of polymerase chain reaction (PCR)-amplified mtDNA is an effective and relatively simple method for species identification of adult specimens of fish species.
Binary character matrices included only restriction sites
(Hillis 1996) from those enzymes that yielded more than one haplotype from all individuals treated.
This result indicates that the polymorphic BamHI restriction site
is outside of the rbcL region and thus the 8.6-kb fragment detected by Hultquist et al.
The PEPCK-luciferase (Luc) construct contains the region from -592 (NheI restriction site
) to +87 (Bg/II restriction site
) of the rat proximal PEPCK promoter ligated into the pGL3-basic vector (Promega, Madison, WI), as previously described (8).
Data presented in Cronin (1992) were shown to differentiate the Alaska, British Columbia, and Oregon black-tailed deer mtDNA haplotypes from the mule deer and western white-tailed deer haplotypes by 25 restriction site
changes, whereas the greatest number of restriction site
changes within the clade which included western white-tailed deer and mule deer was six restriction site
Further investigation (Skuder et al., 1995) revealed that this marker is a mitochondrial polymorphism, resulting from the loss of a MspI restriction site
(MspI-) at position 15,925 of the mitochondrial genome (Anderson et al., 1981).
We have conducted simultaneous and separate parsimony analyses of two data sets, one comprising a miscellaneous set of "structural characters," representing variation in morphology, anatomy, biochemistry, and structural sequences of the chloroplast genome, the second reflecting cpDNA restriction site