restriction enzyme


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

restriction enzyme

n.
Any of a group of enzymes that catalyze the cleavage of DNA at specific sites to produce discrete fragments, used especially in genetic engineering. Also called restriction endonuclease.

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

One of the many enzymes that break DNA at specific sites. These enzymes are extensively used in research and in GENETIC ENGINEERING. Also known as restriction endonucleases.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.
References in periodicals archive ?
The HAdV-4 isolate was genome typed as variant 4a1 by both in vitro and in silico restriction enzyme analyses (Table 2).
Revers 25 bp with EcoR1 restriction enzyme site: Reverse: 5' GAA-TTC-GCC-CCC-ATA-TCC-TAC-TGG-C 3'
It can be seen that the positive control has only one band which follows and agrees with the results of Gatphayak et al [3] who stated that the mutation would render the said restriction enzyme inactive due to SNPC119T.
oxysporum and other formae speciales and the identification of race 1 isolates with specific restriction enzymes (Rimondi et al., 2010).
The restriction enzyme has to be added just once and it autonomously finds molecules which have to be cut.
monocytogenes strains was determined by PFGE of DNA digested with the restriction enzymes ApaI and SmaI, and electrophoresed on a Chef DRII system (Bio-Rad Laboratories, Hercules, CA).
(iii) Digest pFUSEss-CHIg-hG1 and PCR-amplified [V.sub.H] separately using the same FastDigest restriction enzymes (e.g., EcoRI and NheI) and pFUSE2ss-CLIghk plasmids and PCR-amplified [V.sub.L] separately using the same FastDigest restriction enzymes (e.g., EcoRI and BsiWI).
A DNA and restriction enzyme implementation of Turing machines.
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining.
Then introduce the concept that a restriction enzyme works by "chopping" DNA into smaller pieces (restriction enzymes can be thought of as "scissors" that cut DNA).
Restriction enzyme digestion was performed using Sal I, Ban II (37[degrees]C) and Taq I (65[dergees]C) enzymes, each reaction was performed either with 17 [micro]l rDNA, 2 [micro]l Multi Core buffer and 1 ul enzyme restriction (10 U/[micro]l) to a 20 [micro]l final volume.
Restriction digests were performed with the purified PCR product in a 40 [micro]L reaction with approximately 1200 ng of DNA (24 [micro]L), 2 [micro]L of restriction enzyme, 4 [micro]L of restriction enzyme 10X buffer, and 10 [micro]L of sterile mili-Q distilled [H.sub.2]O.

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