restriction endonuclease


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.
References in periodicals archive ?
The discovery of restriction endonucleases, one class of deoxyribonucleases, in the late 1960s sparked a revolution in the study of variation at the molecular level.
It is likely that many of the 12 restriction endonucleases utilized did not yield differing haplotypes because these areas of the amplicon are highly conserved.
Purified mtDNA was digested using 13 restriction endonucleases, under conditions suggested by the supplier (New York Biolabs, Inc.) for 7 h at 37 C.
Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.
To construct the physical map of the chromosome of Psm, three rare-cutting restriction endonucleases were chosen based on G+C content of P syringae strains, several enzymes with A+T rich recognition sequences were tested, among these enzymes PacI (TTAATTAA), PmeI (GTTTAAAC) and SwaI (ATTTAAAT) were selected which originated 14, 15 and 16 fragments, respectively.
The Stx-2A bacteriophages were highly similar in morphologic features, restriction endonuclease profiles, chromosomal integration sites, and superinfection immunity (2,3) and showed <65% similarity to Stx phages from non-O104 strains.
Generally, to produce sticky or complementary ends for sticky-end DNA cloning, insert DNA and vector are separately cut with same restriction endonuclease enzymes.
A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data.
1A in the Data Supplement that accompanies the online version of this brief communication at http://www.clinchem.org/content/vol57/ issue5, genomic DNA is first digested by a restriction endonuclease (RsaI in this case) into fragments, one of which contains the entire target region between 2 closely spaced restriction sites.
The amplified fliC gene was cleaved with HhaI restriction endonuclease (Invitrogen), when fliC(M) primers were used, and RsaI restriction endonuclease (Invitrogen), when fliC(F) primers were used.

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