The discovery of restriction endonucleases
, one class of deoxyribonucleases, in the late 1960s sparked a revolution in the study of variation at the molecular level.
It is likely that many of the 12 restriction endonucleases
utilized did not yield differing haplotypes because these areas of the amplicon are highly conserved.
cleavage maps of animal mitochondrial DNAs.
Purified mtDNA was digested using 13 restriction endonucleases
, under conditions suggested by the supplier (New York Biolabs, Inc.) for 7 h at 37 C.
Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases
with infrequent recognition sites.
To construct the physical map of the chromosome of Psm, three rare-cutting restriction endonucleases
were chosen based on G+C content of P syringae strains, several enzymes with A+T rich recognition sequences were tested, among these enzymes PacI (TTAATTAA), PmeI (GTTTAAAC) and SwaI (ATTTAAAT) were selected which originated 14, 15 and 16 fragments, respectively.
The Stx-2A bacteriophages were highly similar in morphologic features, restriction endonuclease
profiles, chromosomal integration sites, and superinfection immunity (2,3) and showed <65% similarity to Stx phages from non-O104 strains.
Generally, to produce sticky or complementary ends for sticky-end DNA cloning, insert DNA and vector are separately cut with same restriction endonuclease
A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease
mapping and DNA sequence data.
1A in the Data Supplement that accompanies the online version of this brief communication at http://www.clinchem.org/content/vol57/ issue5, genomic DNA is first digested by a restriction endonuclease
(RsaI in this case) into fragments, one of which contains the entire target region between 2 closely spaced restriction sites.
The amplified fliC gene was cleaved with HhaI restriction endonuclease
(Invitrogen), when fliC(M) primers were used, and RsaI restriction endonuclease
(Invitrogen), when fliC(F) primers were used.
Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease
assay on the quality characteristics of commercial pork loins.