Positive clones were then further tested by restriction digest
With the array, any reference sample can be used for analysis without changes to the standard aCGH protocol, and no restriction digest
The restriction digest
products were electrophoresed on 2% agarose gel in 1X TAE buffer and PCR-RFLP bands were visualized under UV light by ethidium bromide staining.
To investigate the fragment length-dependent sequencing bias, we prepared a restriction digest
of [lambda] DNA (Invitrogen).
Our PCR, restriction digest
, and electrophoresis protocols were adapted from Gruntzig et al.
1997), when applied properly, differentiates domestic cats from cougars based on examining the RsaI restriction digest
Four distinct phylogenetic lineages of human serotype III GBS have been identified by PFGE of restriction digest
patterns and designated restriction digest
patterns type III-1, III-2, III-3, and III-4 (11).
Fragment lengths were estimated using a HaeIII digest of [phi]X174RF (New England Biolabs) and an appropriate restriction digest
of strain 1A2 as lane markers.
To quantify the proportion of the DNA that could be digested, we divided the density of the full-length PCR product after the restriction digest
by the density before the restriction digest
pseudomallei were typed by PFGE of an XbaI restriction digest
of bacterial chromosomal DNA (Figure).
The mean mitochondrial genome size was calculated by averaging the sum of the individual fragments for each restriction digest
An aliquot of the restriction digest
is taken for PFGE analysis as a control for DNA preparation; the remaining prepared DNA molecules are analyzed with the DLA system.