Another possible way to measure manually the C[O.sub.2] produced during the biodegradation of polymers is by the use of a Bartha respirometer
, which was originally developed for determining the biodegradation of pollutants in soil (40-43).
Because sandbar sharks are continuously active, we chose to measure SMR in immobilized and artificially ventilated animals in flow-through respirometers
. As a check of this technique, a two-point power-performance curve was constructed by using the logarithms of self-paired SMR and RMR and the mean swimming speed of the animal in the annular respirometer
"Blank" experiments, without fish in the respirometers
, were performed to correct for potential microbial oxygen consumption.
for control of the activated sludge process are furnished by Arthur Technology; Bioscience, Inc.; N-Con Systems Co., Inc.
Biochemical Research Equipment: DNA Synthesizers, Enzyme Analyzers, Respirometers
After metabolic rate was measured during byssus attachment and SDA, mussels were removed from the chambers but maintained in their cups and placed in an aquarium that received the same flow-through seawater as used in the respirometers
. Four days later the mussels were put back into the respiration chambers and left for 4 days to settle before oxygen consumption was measured to determine the standard metabolic rate.
The allometric (size-dependent) influence on standard metabolic rate (SMR) in juvenile sandbar sharks was recently determined over the entire size range (42-92 cm PCL, 1-10 kg) characteristic of the Chesapeake Bay nursery area in flow-through respirometers
for sharks treated with a neuromuscular blocker (Dowd et al., 2006).
A total of 150 shrimp, with averages of 1.27 [+ or -] 0.4 g and 1.10 [+ or -] 0.36 cm, was used for the routine metabolism measure utilizing sealed respirometers
. Ten shrimp with 3 replicates were subjected to the oxygen consumption measure in 1 of the 4 concentrations (control; 0.02, 0.04, 0.08 and 0.133 mg [L.sup.-1]) of Hg.
Five pieces of Elodes were placed in five respirometers
and left under one light for six hours.
For the lower temperatures (10[degrees] and 15.5[degrees] C), the respirometers
were placed in water baths to which ice was gradually added until the test temperature was attained.
were made of clear plastic and varied in size (85, 120, and 700 c[m.sup.3]) for use with different-sized animals.
Indirect calorimetry was accomplished with oxygen electrodes (Nester Inc., non-oxygen-consuming) in fixed-volume, closed, Plexiglas respirometers
at the ambient temperature (6-9 [degrees] C) and salinity of collection; filtered (1.0 [[micro]meter]) seawater was used.