The detection of beta-lactamase-producing plasmid types (Africa, Asia, and Toronto), tetM tetracycline resistance plasmid
types (America and Dutch), and the determination of the mutation Ser-91 in gyrA was performed by PCR using specific primers followed by HinfI digestion of tetM and gyrA products as previously described [9, 11].
The plasmid curing using temperature and SDS causes elimination of resistance plasmid
and revert the resistant strains into susceptible form.
Characterization of endemic Shigella flexneri strains in Somalia: antimicrobial resistance plasmid
profiles and serotype correlation.
Monitoring of the resistance plasmid
also provides some epidemiological information which is useful to trace emerging resistance and multidrug resistance.
coli, sequence-based gene prediction: annotation of a resistance plasmid
, and nucleic acid structure prediction: polymerase chain reaction and RNA interference.
Emergence of tetracycline resistance due to a multiple drug resistance plasmid
in Vibrio cholerae O139.
Elimination of plasmids by curing is an essential step in procedures used to investigate to be reservoir resistance plasmid
or chromosom .
When resistance plasmid
transfer failed in mating experiments, a transformation was used.
1987 Persistance of an antibiotic resistance plasmid
in intestinal Escherichia coli of chickens in the absence of selective pressure.
The acquisition offosA3 by a successful clone and an efficient resistance plasmid
, which might entail subsequent dissemination and alerts to the need of close monitoring of fosfomycin resistant isolates, is of particular concern.
Although the patient in Pennsylvania lacked a relevant travel history, the timeline and structural similarities of these fosA3-carrying plasmids make it plausible that this multidrug resistance plasmid
originated in E.
This truncation was previously described on the multiple antimicrobial drug resistance plasmid
pRMH712 isolated from strain 4003 (ENA accession no.