A blue compound used as a redox indicator in the reductase test of milk and also as a pH indicator (orange at 3.8, violet at 6.5).
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The microplates were incubated at 37 [degrees]C for 24 h; then, 30 [micro]L 0.02% resazurin (Sigma-Aldrich) aqueous solution was added to every well (Sarker et al., 2007).
Kumarasamy, Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals, Methods, 42, 321 (2007).
The metabolic conversion of resazurin to resorufin was used as a measure for viability, which was estimated 1 h and 24 h post-treatment with AAPH and UV radiation, respectively.
The viability of cells in individual wells was assessed by adding 20 [micro]L of resazurin toxicology reagent (Sigma-Aldrich) per well and measuring fluorescence intensity (exc.
For enrichment culture and isolation, the medium was prepared based on the modification by Sowers and Schreier [17] and contained the following compounds (per liter distilled water): K[H.sub.2]P[O.sub.4], 0.51 g; [K.sub.2]HP[O.sub.4], 0.24 g; MgS[O.sub.4] x 7[H.sub.2]O, 0.204 g; [(N[H.sub.4]).sub.2]S[O.sub.4], 0.255 g; NaCl, 1.02 g; Ca[Cl.sub.2] x 2[H.sub.2]O, 0.134 g; vitamin solution, 10.0 mL; yeast extract, 1.0 g; sodium acetate, 50 mM; methanol, 50 mM; sodium formate, 50 mM; rumen fluid, 300 mL; NaHC[O.sub.3], 5.0 g; resazurin, 0.001 g; 0.2 M cysteine hydrochloride, 15 mL; and 0.2 M [Na.sub.2]S x 9[H.sub.2]O, 4.0 mL.
Several approaches are possible to determine the cytotoxicity of material like LDH release, MTT formation, XTT formation, neutral red uptake, kenacid blue binding, acid phosphatase activity, sulforhodamine B binding and resazurin binding (20).
The medium used for isolation and cultivation of strains grown on Thioglycolate Agar (TGA) medium and was prepared as follows; 15 g/L pancreatic digest of casein, 5.5 g/L dextrose, 5 g/L yeast extract, 2.5 g/L sodium chloride, 0.5 g/L sodium thioglycolate, 0.5 g/L L-cystine, 1 mg/L resazurin, 75 g/L agar with final pH 7 (29).
After the incubation, 20 [micro]L of resazurin dye for each well was added and, then, the colorimetric reaction was observed.