To each well 10 uL of resazurin
indicator solution was added.
Medium nutritious dry: medium thioglycolic with resazurin
The viability of cells was confirmed using resazurin
dye after 5 h incubation, and the MIC was recorded for each extract.
ADP-ribosyl cyclase, 8-Br-cADPR, alcohol dehydrogenase, resazurin
, NAD, nicotinamide, bovine serum albumin (BSA), diaphorase, 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide (MTT), bicine, alkaline phosphatase, riboflavin 5'-mono-phosphate (FMN), NADase, nucleotide pyrophosphatase, and ethylene diamine tetraacetic acid (EDTA)-Na were purchased from Sigma (St.
Microtitre plate-based antibacterial assay incorporating resazurin
as an indicator of cell growth and its application in the in vitro antibacterial screening of phytochemicals.
2] stream in 300ml of incubation solution, without resazurin
and micromineral solution, using HCl-cysteine as the reducing agent (MOULD et al.
2] saturation until adding the other solutions (buffer, macro- and micro- minerals, resazurin
solution and buffer B) for the culture medium.
For instance, "viability" may be assessed by LDH-release, resazurin
reduction, cell counting or measurement of ATP.
The cytotoxicity of the tested samples was performed by resazurin
reduction assay as previously described (O'Brien et al.
Cellular viability: AlamarBlue[R] cell viability reagent functions as a cell health indicator by using the natural reducing power of living cells to convert resazurin
to the fluorescent molecule, resorufin, as a cell viability indicator.
microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.
First, culture medium was replaced with a medium containing 10% of resazurin