In the ActF channel, reptilase
and factor XIII are used to produce a clot with no platelet contribution.
The hemotoxin batroxobin, also known as reptilase
, was added to activate BMAC (from the iliac crest) to produce an adhesive clot.
Snake venom thrombin-like enzymes: from reptilase
This band corresponded to the presence of residual fibrinogen (1), because it disappeared after reptilase
A normal reptilase
time despite PTT >180 seconds (Table 1) eliminated dysfibrinogenemia as the cause of the coagulopathy and indicated the presence of heparin.[sup.11] Serum fibrinogen was normal, indicating that his liver was producing normal amounts of fibrinogen.
This is achieved by adding reptilase
(batroxobin), which generates fibrin without platelet activation, and factor [XIII.sub.a] which cross-links fibrin monomers.
Currently, several thrombolytic agents such as streptokinase, urokinase, prourokinase, reteplase (r-PA), alteplase (t-PA), reptilase
, brinase and anisoylated purified streptokinase activator complex (APSAC) are available for clinical use (1).
We then performed PT; activated partial thromboplastin time (APTT); fibrinogen (clottable and immunological); d-dimer; reptilase
time; thrombin times; coagulation factors II, V, VII, VIII, IX, X, XI, XII; anti-thrombin (AT); alpha-2 antiplasmin (AP); protein C (PC); mixing studies (using rabbit normal plasma); lupus anticoagulant screening and confirmatory testing (dilute Russell's viper venom [American Diagnostica, Greenwich CT], STACLOT LA, [Diagnostica-Stago, Inc., Parsippany NJ] ); and plasminogen using reagents from Diagnostica-Stago, Inc.
At 2.5 m/s the Welch f-test identified significant changes for platelets, monocytes, batroxobin test (Reptilase
time), D-dimer fragments, antithrombin, potassium, phosphorus, magnesium, ASAT, ALAT, lipase, and LDH.
A 49-year-old African-American female with history of hepatitis C and hypertension was investigated for a possible dysfibrinogenemia when an abnormal thrombin time (TT) and reptilase
time (RT) were reported by the laboratory.
The fibrin contribution to the maximal clot strength ([MA.sub.fibrin]) is quantified by the addition of an activator (reptilase
and activated factor XIII), which facilitates the formation of the cross-linked fibrin clot.