Since most synthetic biology works use the GFP reporter, and moreover, GFP provided sufficient resolution to analyze the promoters and also allowed for single-cell analysis (a possible calibrator approach), we focused on the section on the pMR1 reporter system containing the short-lived variant of the reporter protein
GFP, a reporter protein
in negative-sense, are flanked by the 3'- and 5'-noncoding region of the NS RNA segment of influenza A/WSN/33 virus  placed between a truncated human RNA polymerase I promotor (POLI) and hepatitis delta virus ribozyme (R).
After incubation, the wells were rinsed repeatedly with washing buffer, and the PAD activity of the captured enzymes was assessed using fibrinogen as a reporter protein
. For this assay, 100 ng of fibrinogen (F3879 Sigma, St.
3C shows as the simultaneous presence of sub-inhibitory concentration of levofloxacin and increasing concentrations of curcumin have no influence on the relative activity of the reporter protein
When implanted into animals, these cells express the reporter protein
in a HIF-1-dependent manner, and the amount of reporter protein
expression can be measured using a molecular probe that binds to or is metabolized by these proteins.
When ligands are added to the system, the receptor is activated and there is consequent production of reporter protein
which can be measured.The routinely used reporter genes whose products can be assayed easily include luciferase, beta-galactosidase, or green fluorescent protein.
The only reported study using a fluorescent reporter protein
(enhanced green fluorescent protein (EGFP)) in a haliotid species was during the transfection of ova, embryos, and sperm in Haliotis discus hannai by Wang et al.
A novel yellowish-green fluorescent protein from the marine copepod Chiridius poppei, and its use as a reporter protein
in HeLa cells.
These cells then produce Gluc mRNA which is translated into the reporter protein
. Isolation of EVs from these cells will provide EVs containing the reporter protein
from the cell.
To study the promoter and enhancer control of gene expression, firefly luciferase is widely used as a reporter protein
because it has high sensitivity and a broad linear range.
The signaling capacities of both AP and acridinium were found to be damaged by addition of sulfhydryl groups; therefore, conjugates using these materials were prepared with maleimide-modified reporter protein
alternating with sulfhydryl-modified BSA.