reporter gene


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Related to reporter gene: Marker gene

reporter gene

, reporter
A gene whose products are easily detected or measured. In biochemical research it is linked to a gene of interest and used to signal to researchers that the gene has been successfully inserted into a cell or chromosome.

reporter gene

a test GENE with a coding region whose PHENOTYPE can be easily detected. Ideally it should be possible to assay the phenotype quantitatively. The reporter gene may be fused immediately DOWNSTREAM of a regulatory region so that its expression (see GENE EXPRESSION is under the influence of that region. The extent and pattern of expression of the reporter gene construct, determined by assay of phenotype, can be used to analyse gene regulation.
References in periodicals archive ?
donovani cell lines expressing firefly luciferase reporter gene (luc.) have been developed as a part of episomal vector and suitability of these cell lines for in vitro screening of antileishmanial agents has been establised (52).
Luciferase Reporter Gene Assay--Transient transfection was performed as described by the Fugene 6 manufacturer's protocol.
Fusion of the isolated promoters to the uidA reporter gene and use of transient GUS expression assays proved an effective method of confirming each promoter's activity in sugarcane and wheat cells.
Which potential benefit is associated with multiple reporter gene engineering?
If this assumption is valid, luminescence measurements directly reflect reporter gene transcriptional activity.
(1991); additionally, the study showed that the 2.2-kb hGFAP promoter drives expression of a LacZ reporter gene in mice in a manner similar to that of endogenous GFAP (Brenner et al., 1994).
Chen et al., "Tyrosinase as a multifunctional reporter gene for photoacoustic/MRI/PET triple modality molecular imaging," Scientific Reports, vol.
Reagents and assay kits include reporter gene assays, second messenger assays, cell growth assays, cell death assays and other assays.
The assay, integrating the luciferase reporter gene technology, is an all-inclusive platform that incorporates two optimised media to be used during cell culture and in diluting test samples, a reference agonist, Luciferase Detection Reagent and a cell culture-ready assay plate.
In SIF-seq, hundreds or diousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.
The [bla.sub.TEM-1] gene has been placed under the control of the LexA-binding box and used as reporter gene. The expression of TEM-1 [beta]-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8 [micro]g/ml, reduced dramatically the expression of the reporter gene.
To construct reporter plasmids, we used the pGL3-Basic vector, in which two random cis-elements, 5xGAL4 and 3xDRE, and a minimal TATA promoter [27] were cloned in the MCS before luciferase reporter gene. The 35S::DBD- (GAL4-DNAbinding domain-) CMYB1 effector plasmid was constructed by inserting DNA fragments containing CMYB1 coding region and GAL4-DNA binding domain sequence of pGBKT7 vector (Clontech, USA) into the Spe I and Xba I sites of pA7.