In this study, just IncFI[I.sub.Y] plasmid replicon
were detected mostly in strains of the pathogenic biotype 4 isolated from human diarrheic feces; however, it did not relate to any acquired resistance genes (Table 1).
Recently, a shuttle vector (pYCJ) based on the SNJ1 replicon
(nucleotides 1-4481) was also constructed and validated for the expression and purification of heterologous proteins .
Optionally, the gene targeting system with geminivirus replicons
includes an additional donor DNA template.
Currently, HCV sub- or full-length genomic replicon
or infected cells were commonly used to analyze drug-resistance profile of DAA , and there are several methods to evaluate the emerged drug-resistance .
All the transformants were subjected to PCR based replicon
typing (5 multiplex and 3 simplex PCR), and it was observed that F inc (n=58) type was more prevalent, followed by K/B (n=40) and Y (n=13), I (n=17) and P (n=7) inc type was also observed in E.coli isolates.
Siddiqui, "Hepatitis C virus subgenomic replicons
induce endoplasmic reticulum stress activating an intracellular signaling pathway," Journal of Virology, vol.
In in vitro studies, Daklinza has shown anti-viral activity across genotypes 1-6, with EC50 values from picomolar (pM) to low nanomolar (nM) against wild type replicons
A replicon-based PCR typing of plasmid incompatibility groups (Inc/rep) was performed using previously published protocols (11,12) with 5 multiplex and 6 single reactions recognizing the FIA, FIB, FIC, HI1, HI2, I1-Iy, L/M, N, P, W, T, A/C, K, B/O, X, Y, F, FIIA, and Q replicons
of the vectors pNZ124, pNZ2105, pNZ2103, pNZ7021, pNZ2122, and pNZ2123 are identical and came originally from the Lactococcus lactis plasmid pSH71.
MCM2 was found to get dissociated from replicons
shortly after their initiation .
In sections on genes and chromosomes, DNA replication and recombination, transcription and post-transcriptional mechanisms, and gene regulation, they consider such topics as the content of the genome, clusters and repeats, extrachomosomal replicons
, RNA splicing and processing, and phage strategies.
Cytopathogenic and noncytopathogenic RNA replicons
of Classical swine fever virus.