Specific inhibition of bovine viral diarrhea virus
replicase.
However, recent work with JHF1 viral RNA suggested that its 5'-NTR (+) does not contain RNA packaging signals (14) and authors further speculate that it may reside in the RNA region encoding the
replicase.
Virions are enveloped and highly pleomorphic, with 50 to 200 nm in diameter, and a positive-sense ssRNA as genome, in which the 5' two-thrids (ORFs 1a and 1b) code for the
replicase complex, highly conserved among members in each of the 3 groups the genus is divided, while the 3' final third codes for the structural proteins [2].
El Automated Q-Beta
Replicase Amplification Assay[R] amplifica la subunidad 23 del ARN ribosomico de las micobacterias del complejo MTB; la sensibilidad ha sido del 84% y la especificidad, del 97%; permite obtener resultados en un maximo de 6,5 horas.
In the first section, they focus on the molecular biology of the virus itself and topics including coronavirus entry,
replicase gene function, reverse genetics, genome packaging, and molecular evolution.
Insertion of EGFP into the
replicase gene of Semliki Forest virus results in a novel, genetically stable marker virus.
The SARS-CoV has a RNA genome of 30 Kb encoding the
replicase, spike glycoprotein, envelop protein, membrane protein, and nucleocapsid (2-4).
Resistance to wheat streak mosaic virus in transgenic wheat expressing the viral
replicase (NIb) gene.
For the study, he and his associates developed PCR probes to target regions of the
replicase la gene that are conserved among genetically diverse animal and human coronaviruses (J.
For the study, he and his associates developed PCR probes to target regions of the
replicase 1a gene that are conserved among genetically diverse animal and human coronaviruses (J.
Moreover, the requirement of full-cDNAs, in vitro transcription of each one of the genomic RNAs and mix-and-match of each component along with the viral
replicase makes this sort of experiments technically very difficult to perform.
Ithaca, NY) has patented a virus-induced resistance that may be transferred from one plant generation to another in which transgenic plants containing a coding sequence, taken from the read-through portion of the
replicase portion of the viral genome, are resistant to subsequent disease by the virus.
