Many episodes of
reoxygenation were estimated to greatly prolong the operation.
Neuroprotection in rat hippocampal slices subjected to oxygen and glucose deprivation followed by a
reoxygenation period was also demonstrated by galantamine.
Several NIRS studies have shown that the muscle
reoxygenation rate after either static (Fryer et al., 2015) or dynamic (McLean et al., 2016) exercise could be an important determinant of both muscle fatigue and training status.
lucidum protected rat cerebral cortical neurons from injury induced by hypoxia/
reoxygenation ex-posure (Zhao et al., 2004) as well as reduced neurological deficits in rats after cerebral ischemic injury (Zhou et al., 2010).
Fractionation is used to allow for repair of normal tissue, and it allows for
reoxygenation of tumor cells making them more susceptible to the next dose of radiation therapy.
Furthermore, short periods of anoxia (3h) followed by
reoxygenation (0-5 h) led to a time-dependent increase of caspase activity in human umbilical vein endothelial cells (HUVECs), which was associated with a significant decrease in glucosylceramide synthase mRNA levels and protein expression, but no changes in SMase.
These findings would fit with a chemoradiation-related tumor
reoxygenation phenomenon [34].
In our preliminary experiments, we established that the combination of 4 hours of hypoxia at 0.2% O2, combined with glucose and serum deprivation and followed by
reoxygenation in normoxic culture conditions with serum and glucose, is sufficient for reproducibly inducing significant cell loss.
For
reoxygenation, plates were removed from the anoxic chamber to a normoxic chamber for 2 h.
Furthermore, Hill detected an increased intranuclear gelatinolytic activity immediately after
reoxygenation in a primary culture of cortical neurons after oxygen and glucose deprivation [16].
However, remarkable differences occurred in the recovery kinetic variables (Table 2 and Figure 4); i.e., in the healthy subject, the rate of
reoxygenation was substantially faster in each leg than in the patients, in both NP and CP (Figure 4).
For
reoxygenation, following hypoxic conditions, the medium was replaced with fresh medium and the cells were kept in normoxic conditions.
