MR-VP medium was used for methyl red test
. The sterile glucose broth was inoculated with the test culture 7ml and incubated at 37[degrees]C for 24hr.
Biochemical characterization of the bacterial isolates were done by starch hydrolysis test, citrate utilization test, litmus milk reaction, methyl red test
, voges, proskauer test, catalase test following same procedures as described previously in manual using fresh bacterial culture (Cappuccino and Sherman, 2002).
Further, characterization of the bacterial strain was performed based on oxidase test, hanging drop technique, mannitol fermentation test, nitrate reduction test, TSI test and methyl red test
Species identification and differentiation of enteric coliforms of the family Enterobacteriaceae was made on the basis of their biochemical properties and enzymatic reactions in the presence of specific substrate from the results of biochemical tests like Indole Production test, Methyl Red test
, Voges- Proskauer test, Citrate Utilization test, Urease test, H2S Production test, Nitrate Reduction test and Litmus Milk test (Table 5 to Table 13) (Fig.
Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test
(MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured.
As he speaks, engineers brave the mercury at minus 15 degrees Celsius (5 deg F) to descend onto the ice, drilling holes to inject harmless red test
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The isolate was found to give positive results for Indole Test, Citrate Utilization Test, Urease Test and negative for Methyl Red Test
, Voges Proskauer Test.
IDENTIFICATION: Late lactose fermenting gram negative bacilli isolated from various clinical samples were identified by conventional biochemical tests (Triple sugar iron agar test, Methyl red test
, Voges Proskauer test, indole test, citrate utilization test; phenyl alanine agar test, urea hydrolysis, lysine iron agar test, arginine utilization test, ornithine utilization test, O nitrophenyl [beta] D galactopyranoside test, (1) and Vitek 2 system.
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Then, put the mixed powders into a test tube (10 mm x 100 mm) with a wettish congo red test
paper located at 2 cm above the sample so that the distance can help to avoid being influenced by HCl which is released from partial PVC decomposition because of high temperature (17).
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