reconstructed ion chromatogram

reconstructed ion chromatogram

A normal mass spectrometric data set consisting of full mass spectra recorded sequentially in time as a sample is passed to the ion source, either from a direct-insertion probe or from a chromatograph (e.g., GC/MS). The total ion current trace is the sum of ion abundances in each mass spectrum plotted vs time.

Each spectrum contains a pattern of molecular ions and fragment ions, which can be specified, and data plotted of scan-by-scan abundance of specific ions vs time—the so-called reconstructed ion chromatogram (RIC). The RIC can be used to identify all ions belonging in a single mass spectrum, based on their coincident peaks in time, while discriminating against background ions, and can be used to screen a GC/MS run for related classes of compounds by re-constructing ion chromatograms for common structurally specific ions.
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Abstract: Mass spectra of reconstructed ion chromatogram (MRIC) technique are used to follow up the grafting copolymerization of 1-vinyl-2-pyrrolidinone (VP) 1-vinyl-3-anisylidine-2-pyrrolidinone (VAP) and ethylmethacrylate (EMA) and cellulose extracted from kenaf using xanthate method.
3, B, D, and F, shows reconstructed ion chromatograms (RICs) appropriate to monohydroxycholesterols and hydroxycholest-4-en-3-ones (HC/HCO, m/z 539.437/ 534.405), 30-HCA/3O-CA (m/z 553.416/548.385), and 3[beta], 7[alpha]-diHCA/7[alpha]H, 3O-CA (m/z 569.411/ 564.380) from O7AHD (upper panels) and control plasma (lower panels) samples derivatized with [[sup.2][H.sub.5]]GP (ii) and [[sup.0][H.sub.5]]GP (i), respectively.
For the quantification of sterols, we recorded only [MS.sup.2] spectra and generated reconstructed ion chromatograms (RICs) for the [[M].sup.+][right arrow] [[M-79].sup.+] transitions over the duration of the LC-[MS.sup.2] run.
Reconstructed ion chromatograms of a blank plasma enriched with IS and therapeutic concentrations of all 8 sulfonylureas are shown in Fig.