, nucleases, and accessory proteins are natural or artificially created tools for gene editing that are used for developing powerful methods of genetic engineering.
polymerase amplification for diagnostic applications.
pCP20###Ampr,Cmr; Flp recombinase
expression###Datsenko and Wanner (2000); Duan et al.
 Nonstandard abbreviations: SFTS, severe fever with thrombocytopenia syndrome; SFTSV, SFTS virus; IFA, immunofluorescence assays; RT, reverse transcription; iNAD, isothermal nucleic acid amplification procedure with bio-optical sensor detection; SMR, silicon microring resonator; RPA, recombinase
polymerase amplification; cDNA, complementary DNA.
However, one has to keep in mind that during in vitro DC development, a relatively late onset of Cre recombinase
occurs, so that the role of CREB in DC differentiation cannot be excluded.
Kaestner and were used to direct expression of cre recombinase
to the gastric mucosa .
expression plasmid is constructed for one-step inactivation of gene encoding pyruvate formate lyase, fumarate reductase, and LacI transcriptional repressor, respectively .
Klemm, "Differential temperature modulation by H-NS of the fimB and fimE recombinase
genes which control the orientation of the type 1 fimbrial phase switch," FEMS Microbiology Letters, vol.
It includes a fragment (named R4') inserted into the umuC gene, which is characterized by a phage integrase (ORF38) and a phage integrase family site-specific recombinase
(ORF37) and encodes 9 genes from different origins with various functions.
Overexpression of RAD51, a recombinase
involved in DNA repair by homologous recombination (HR), is associated with a more aggressive cancer phenotype and treatment resistance in a variety of tumors, including ovarian, prostate, colorectal cancer, and malignant gliomas [6, 7].
Multicopy transgenes are not suitable for conditional knockout; even if transgenes are floxed (loxP sequence from bacteriophage P1 is inserted and can be recombined between two loxP sites by Cre recombinase
from bacteriophage P1), only some of the copies are removed by recombinases
, resulting in the failure of knockout.