Recombinant vector was transformed to E.coli strains (DH5[alpha] and BL21) and positive clones were preceded for plasmid isolation (Figure 1) followed by restriction analysis to confirm successful ligation.
The T4 DNA ligase was engaged for the ligation of PPE17, insert into pET-21b(+) vector, and then
recombinant vector was transformed into E.
Transformation of the TG1 culture with the
recombinant vector was done with heat shock method.
The product type segment is further segmented as attenuated or live Vaccines, Inactivated Vaccines,
recombinant vector & DNA Vaccines, and Others (toxoid, subunit, conjugate vaccines).
Construction of pETSUMO-IFN[alpha]2-T[alpha]1
recombinant vectorThe
recombinant vector was transformed into Escherichia coli and eight positive clones were verified by enzyme digestion.
coli transformed with the pET28a(+) plasmid or the pET28a(+)-PtCacyBP
recombinant vector in response to different salinity challenges.
Accuracy of the
recombinant vector gene sequence and recombinant adeno-associated virus vector infection
The
recombinant vector containing selD gene was transformed according to the manufacturer recommendations (Stratagene).
coli carrying the
recombinant vector were cultured overnight and used for miniprep analysis.
For the SW clone, the 267-bp DNA fragment corresponding to the second rhuIFN[alpha]2 wild-type fragment was cut with BglII and inserted into the pGEX4T1/SynrhuIFN[alpha]2a synthetic full-length
recombinant vector (P2 vector) cut with the same restriction enzyme.