As can be seen in figures 4A and B in all fractions that were analyzed on SDS-PAGE, the overexpression band of the recombinant protein
was deposited in inclusion bodies and no expression band was observed in soluble fraction.
The figure 1 and 2 are related to the SDSPAGE of the recombinant protein
CagA, also for confirmation of this protein the western blot method with Anti-His tag was used.
With the vast developments made in the transferring methods and transient culturing methods, the mass production of recombinant proteins
through transient transferring methods seems viable.
Concurrently,the company has begun an engineering project to convert the 9,000 square foot expansion space within CMF into cell culture and purification suites for production of recombinant proteins
expressed in mammalian cells.
The EPC1 target protein was expressed in [p.sup.PROEXHT-b] vector, with a higher level of expression of the recombinant protein
achieved at 8 h post-IPTG induction at 37[degrees]C.
In order to make the recombinant protein
expressed maximally, we compared the expression conditions as described in "Materials and Methods".
The text reading "As the molecular weight of recombinant BmLeb5 was bigger than the prediction, Learning Content Management System (LC-MS) was performed to test the recombinant protein
molecular weight on TripleTOF[R] 6600 (SCIEX) by Analyst TF 1.7 (AB Sciex) software" should be corrected to "As the molecular weight of recombinant BmLeb5 was bigger than the prediction, liquid chromatography-mass spectrometry was performed to test the recombinant protein
molecular weight on TripleTOF[R] 6600 (SCIEX) by Analyst TF 1.7 (AB Sciex) software."
 Recombinant Protein
Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran;
ReFacto AF (moroctocog alfa)/XYNTHA (Antihemophilic Factor (Recombinant)) is a recombinant protein
drug for treatment and prevention of bleeding in patients with haemophilia A, a rare, chronic, genetic disorder in which the ability of a person's blood to clot is impaired due to missing or reduced levels of a protein known as factor VIII.
To achieve purification, molecular biological methods (cloning and transformation, among others) are used to add an additional tag to the target protein, thereby producing a recombinant protein
. The aim of affinity chromatography is to purify the largest volume of the recombinant protein
was expressed in nine different media (LB ZB 4xZB ZBM ZYB 3xZYB M9 ZYBM9 and 3xZYBM9) by using Isopropyl AY-D-1-thiogalactopyranoside (IPTG) and lactose as inducer.
Expression of recombinant protein
in Escherichia coli (E.