recombinant DNA technology


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recombinant

 [re-kom´bĭ-nant]
1. the new entity (e.g., gene, protein, cell, individual) that results from genetic recombination.
2. pertaining or relating to such an entity.
recombinant DNA technology the process of taking a gene from one organism and inserting it into the DNA of another; called also gene splicing. One commonly used technique involves the insertion of a new fragment of DNA that codes for a specific protein such as human insulin into a bacterium such as Escherichia coli. The gene is first inserted into a plasmid, a self-replicating ring of DNA involved in the transfer of genes between bacteria. The plasmid is cut at a specific site by using a special cutting enzyme called a restriction endonuclease. The same procedure is used to cut out a segment of DNA from another organism, for example, the gene for human insulin. This fragment of insulin DNA is inserted into the plasmid and then sealed into place by an annealing enzyme. The altered plasmid is then taken up by bacterium and incorporated into the genome. When the bacterial cells divide they pass on the new information to the next generation. This produces clones of bacteria that produce large quantities of the new protein, in this example, insulin.



This process has had great impact in the field of medicine. It has revolutionized the manufacture of pharmaceutical products, where it is used to manufacture a variety of proteins used in the treatment of disease, including hormones, vaccines, and interferons.

recombinant DNA technology

Molecular biology The chopping of DNA and moving the pieces, permitting direct examination of the human genome, and identifying the genetic components of various disorders; RDT is also used to develop diagnostic tests, drugs and biologicals for treating disease; the constellation of techniques that comprise 'genetic engineering', in which a gene producing a protein of interest from one organism is spliced into the genome of another organism–eg, a phage DNA integrated into a plasmid is inserted into a 'carrier' bacterium. See Genetic engineering, pBR322, PCR.

recombinant DNA technology (rDNA technology)

all the techniques involved in the construction and manipulation of RECOMBINANT DNA MOLECULES (as in GENETIC ENGINEERING), for the subsequent use and study of such molecules.
References in periodicals archive ?
Genetically modified crops are plants that had their DNA genetically modified using recombinant DNA technology. For example, transgenic maize (WL-73) plants overexpressing the BcWRKY1 gene were generated by Agrobacterium-mediated transformation, which were able to resist 300 mM NaCl stress.
Advantages of recombinant DNA technology in veterinary vaccinology DNA vaccines and cold chain requirement
It is very much possible to discover those genes, controlling milk production and other production traits by using DNA technologies such as DNA mapping, DNA microarray, DNA sequencing and then modify them by genetic engineering or recombinant DNA technology to produce genetically modified organisms (GMO) (Holst, 2009) or Transgenic animals for more and better yield of desired products.
Primarily, scientists use recombinant DNA technology. Using DNA from other sources, researchers will infuse an organism with new DNA to create a hybrid species.
Based in Lexington, MA, Tepha Inc is a developer of medical devices derived from a new class of resorbable polymers that have been engineered utilising recombinant DNA technology.
Background & objective: Recombinant DNA technology allows expression of the human papillomavirus (HPV) major capsid protein (LI) in heterologous expression systems and the recombinant protein self assembles to virus-like particles (VLP).
In an exclusive interview with the A.A, Associate Professor Haydar Bagis from TUBITAK Marmara Research Center (MAM) Institute for Genetic Engineering and Biotechnology said, "development genetic engineering techniques known as recombinant DNA technology allowed scientists to produce genetically modified organisms or transgenic organisms by collecting DNA molecules from different sources and combining them into one molecule to create a new set of genes.
In the early 1970s, Boyer and geneticist Stanley Cohen pioneered a new scientific field called recombinant DNA technology. Upon learning about this development, Swanson placed a call to Boyer and requested a meeting.
Protein Sciences, based in Meriden in Connecticut, focuses on using recombinant DNA technology to develop the next generation of vaccines, therapeutics and diagnostics.
GM, genetic modification, recombinant DNA technology, United Nations, regulation, global warming
Advances in antibody therapeutics began in the 1980s, when recombinant DNA technology initially developed chimeric, then partially human, and finally fully humanized monoclonal antibodies using transgenic techniques.
coli by harnessing recombinant DNA technology. Using some of these lactases, up to 50% of concentrated lactose in whey permeate was converted into galactooligosaccharides as bifidogenic factors and nutraceutical products.

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