random amplified polymorphic DNA

random amplified polymorphic DNA (RAPD)

a technique in which random DNA segments are amplified by the POLYMERASE CHAIN REACTION using single PRIMERS of arbitrary NUCLEOTIDE sequence. The POLYMORPHISMS are detected as DNA segments which are resolved by AGAROSE GEL ELECTROPHORESIS. The pattern of DNA bands generated can be diagnostic for particular STRAINS or SPECIES.
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Applications of random amplified polymorphic DNA (RAPD) in molecular ecology.
The random amplified polymorphic DNA fingerprints (Figure 2) were scored as binary matrix according to the specification of Genalex 6.502 and accounting for missing values wherever found.
The ISSR markers are highly polymorphic and represent a simple, reproducible, efficient and quick method that combines most of the advantages of micro-satellites (SSRs) and amplified fragment length polymorphism (AFLP) to the universality of random amplified polymorphic DNA (RAPD).
Genetic diversity was assessed among 38 chickpea (Cicer arietinum L.) genotypes on the basis of random amplified polymorphic DNA (RAPD) in comparison with agro-morphological traits.
Globally, different DNA techniques including random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLPs), simple sequence repeats (SSR), chloroplast gene rcbL among others have been used to assess the genetic diversities among the populations / some accessions of M.
Random Amplified Polymorphic DNA (RAPD) molecular marker systems used in this study are significantly quicker and simpler in comparison to few other molecular strategies, making the technique famous for evaluating genetic polymorphism in wheat species [11,25].
Detection of genetic diversity among sugarcane (Saccharum spp.) genotypes using random amplified polymorphic DNA markers.
Molecular methods such as isoenzyme electrophoresis, protein sequences, DNA hybridization, restriction fragment length polymorphism (RFLPs) and random amplified polymorphic DNA (RAPDs), have been used as a tools to solve species complexes and/ or analyze genetic variation between species and populations.
Tingey, "Genetic analysis using random amplified polymorphic DNA markers," Methods in Enzymology, vol.
This study was under taken with the objectives to isolate Pseudomonas aeruginosa from various infections like Burns, Chronic Suppurative Otitis Media (CSOM), ventilator associated pneumonia and Catheter associated Urinary tract infections and to find the strain similarities of Pseudomonas by antibiogram, Diene's Mutual inhibition test and Random amplified polymorphic DNA study and to compare the three methods and to evaluate the easy, cheap, and the most suitable method which can be adopted in small laboratories at times of outbreaks.

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