random amplified polymorphic DNA

random amplified polymorphic DNA (RAPD)

a technique in which random DNA segments are amplified by the POLYMERASE CHAIN REACTION using single PRIMERS of arbitrary NUCLEOTIDE sequence. The POLYMORPHISMS are detected as DNA segments which are resolved by AGAROSE GEL ELECTROPHORESIS. The pattern of DNA bands generated can be diagnostic for particular STRAINS or SPECIES.
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In particular, the polymerase chain reaction (PCR) with arbitrary primers is used to analyze the amplification fragment length polymorphism (AFLP), random amplified polymorphic DNA (RADP), and inter-simple sequence repeats (ISSR).
The ISSR markers are highly polymorphic and represent a simple, reproducible, efficient and quick method that combines most of the advantages of micro-satellites (SSRs) and amplified fragment length polymorphism (AFLP) to the universality of random amplified polymorphic DNA (RAPD).
genotypes on the basis of random amplified polymorphic DNA (RAPD) in comparison with agro-morphological traits.
Globally, different DNA techniques including random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLPs), simple sequence repeats (SSR), chloroplast gene rcbL among others have been used to assess the genetic diversities among the populations / some accessions of M.
Random Amplified Polymorphic DNA (RAPD) molecular marker systems used in this study are significantly quicker and simpler in comparison to few other molecular strategies, making the technique famous for evaluating genetic polymorphism in wheat species [11,25].
Molecular methods such as isoenzyme electrophoresis, protein sequences, DNA hybridization, restriction fragment length polymorphism (RFLPs) and random amplified polymorphic DNA (RAPDs), have been used as a tools to solve species complexes and/ or analyze genetic variation between species and populations.
This study was under taken with the objectives to isolate Pseudomonas aeruginosa from various infections like Burns, Chronic Suppurative Otitis Media (CSOM), ventilator associated pneumonia and Catheter associated Urinary tract infections and to find the strain similarities of Pseudomonas by antibiogram, Diene's Mutual inhibition test and Random amplified polymorphic DNA study and to compare the three methods and to evaluate the easy, cheap, and the most suitable method which can be adopted in small laboratories at times of outbreaks.
spiniferus from 7 populations in eastern China using random amplified polymorphic DNA (RAPD).
Thirty nine okra genotypes were assessed for genetic variability using Random Amplified Polymorphic DNA (RAPD) markers.
Identification and genetic mapping of random amplified polymorphic DNA (RAPD) markers to the sheep genome.

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