Between May 2013 and March 2014, radulae
from GKL with shell lengths (SL) between 700 [micro]m and 113 mm were examined.
and copulatory spicules were isolated with warm potassium hydroxide or with sodium hypochlorite (standard household bleach) and thoroughly washed after dissecting anterior and posterior ends.
of more than 100 genera of non-truncatellid rissooideans have been illustrated by various authors; all have a trapezoidal central (rachidian) tooth, as do geomelaniines [ILLUSTRATION FOR FIGURE 6B OMITTED].
were mounted in a water-soluble mounting medium (polyvinyl lactophenol) to make a permanent slide.
were prepared by dissolving buccal masses in 20% KOH at 20-30 [degrees]C, rinsing in distilled water, and mounting on a bed of PVA glue.
Descriptions and illustrations of radulae
and distal genitalia are provided for operculate and stylommatophoran species respectively.
Although the basic design of chiton and limpet radulae
is similar, the radular anatomy of chitons provides a far greater degree of lateral flexibility compared to limpets.
The radula from the single specimen of Paralepetopsis tunnicliffae was mounted for SEM; radulae
of the two other new species were whole-mounted in stain-suffused, nonresinous mounting medium.
were extracted by maceration of the buccal mass in dilute NaOH and then thoroughly rinsed in distilled water.
were prepared from 6 specimens from both the Juan de Fuca Ridge (Tiburon dives: T184 and T458) and the Gorda Ridge (T186 and T188), by dissolving whole bodies in 25% potassium hydroxide.
of postlarvae and small juveniles were removed with a pipette under an inverted microscope following dissolution of tissues by soaking in sodium hypochlorite (0.6% CI concentration; Wako Pure Chemical Industries Ltd, Osaka, Japan) for several minutes.
Formula 1+5+(20-30) (N=4); length up to 53 mm, with up to 57 transverse rows of teeth (but no radulae
from very large specimens available).