The cells were harvested and lysed at 4[degrees]C in radioimmunoprecipitation
assay buffer and 100 x protease inhibitor cocktail (Thermo Scientific, Waltham, MA, USA) for 1h.
We mixed clarified sample supernatants with 55 jL of 10X radioimmunoprecipitation
assay (RIPA) lysis buffer (EMD Millipore Corp.
5 1L protease inhibitor cocktail, and 1455 1L radioimmunoprecipitation
assay buffer (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA).
For Western blot assays, cells were treated with glycitein or DMSO, and cells were harvested using a cell scraper and lysed in radioimmunoprecipitation
assay (RIPA) buffer.
GADA are often detected by radioimmunoprecipitation
assays, although nonradioactive formats are available.
Briefly, cells were lysed in 1 mL ice-cold radioimmunoprecipitation
assay buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) supplemented with a protease inhibitor mixture (Roche Applied Sciences, Indianapolis, IN, USA) for 10 min.
Positive samples were confirmed using a quantitative radioimmunoprecipitation
To produce a test that could be adapted for routine use, a radioimmunoprecipitation
assay (RIPA) was developed.
cruzi is enzootic in domestic dogs and wild canids in some areas of the United States where foxhounds were tested, all samples that yielded Leishmania IIF titers >128 were tested in the radioimmunoprecipitation
assay (RIPA) for T.
CHATTAHOOCHEE RIVER NATIONAL RECREATIONAL AREA (longest, at 20 of each) JACOBIAN ELLIPSOID OF EQUILIBRIUM (15) OED POLLAIUOLESQUE-BOTTICELLESQUE (14) OED Botticellian 1939, AEGIRITE-AKERMANITE-MICROCLINE (14) OED innelite 1963 RADIOIMMUNOPRECIPITATION
Although the Western blot is the most common confirmatory test, others are sometime used, including the indirect fluorescent antibody assay (IFA) and the radioimmunoprecipitation
In 1985, the CDC introduced recommendations for the screening of blood and plasma to detect HIV; these recommendations call for an enzyme immunoassay and, if the results of this first assay are positive, a repeated enzyme immunoassay and then confirmation by either immunofluorescence, radioimmunoprecipitation
, or Western blot if the results are repeatedly positive.