radioimmunoassay

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radioimmunoassay

 (RIA) [ra″de-o-im″u-no-as´a]
a sensitive assay method that can be used for the measurement of minute quantities of specific antibodies or any antigen, such as a hormone or drug, against which specific antibodies can be raised. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit, and hormone that has been labeled with a radioisotope. These are mixed with the assay specimen and the antigen (hormone) bound to antibody is separated from the unbound antigen by chromatography or other means. Because any hormone in the assay specimen competes with the radiolabeled hormone for antibody binding sites, the amount of hormone in the specimen is inversely proportional to the radioactivity of the bound fraction or directly proportional to the activity of the free fraction. This is the standard method for clinical laboratory measurements of hormones and is also used for therapeutic drug monitoring, drug abuse screening, and other laboratory tests.

ra·di·o·im·mu·no·as·say (RIA),

(rā'dē-ō-im'yū-nō-as'sā),
An immunologic (immunochemical) procedure that uses competitive inhibition between a radioisotope-labeled antigen or other substance and unlabeled antigen for antiserums, binding as a means to quantify the agent of interest; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants. Minute quantities of enzymes, hormones, or other substances can be assayed.

radioimmunoassay

/ra·dio·im·mu·no·as·say/ (-im″u-no-as´a) a highly sensitive and specific assay method that uses the competition between radiolabeled and unlabeled substances in an antigen-antibody reaction to determine the concentration of the unlabeled substance, which may be an antibody or a substance against which specific antibodies can be produced.

radioimmunoassay

(rā′dē-ō-ĭm′yə-nō-ăs′ā, -ĭm-yo͞o′-)
n. Abbr. RIA
A technique used to quantitate minute amounts of a substance, such as a hormone, in blood or another liquid. It involves the use of radioisotopes and of antibodies to the substance being investigated.

radioimmunoassay (RIA)

[rā′dē·ō·im′yənō·as′ā]
Etymology: L, radius + immunis, free; Fr, essayer, to try
a technique in radiology used to determine the concentration of an antigen, antibody, or other protein in the serum. The technique involves the injection of a known amount of a radioactively labeled substance that reacts with the protein in question.

radioimmunoassay

see RIA.

ra·di·o·im·mu·no·as·say

(RIA) (rā'dē-ō-im'yū-nō-as'sā)
An immunologic (immunochemical) procedure that uses the competition between radioisotope-labeled antigen (hormone) or other substance and unlabeled antigen for antiserums, resulting in quantitation of the unlabeled antigen; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants.

radioimmunoassay

Any method of measuring the extent of linkage between ANTIGEN and ANTIBODY in which one or other of these is labelled with a radioactive substance (radionuclide). Measurement of radiation can be remarkably precise.

radioimmunoassay

reaction of a radioisotope-labelled agent with specific antibodies; allows light microscopy visualization of antigen-antibody complexes in tissue samples

radioimmunoassay

a sensitive assay method used for the measurement of minute quantities of specific antibodies or any antigen, such as hormones or drugs, against which specific antibodies can be raised; abbreviated RIA. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit. In the assay either the antigen or antibody is labeled with radioisotope.
References in periodicals archive ?
We based the 30-min time point for blood collection on previous toxicokinetic experiments to assure measurable levels of toxin in fractionated plasma by radioimmunoassay (RIA) (Woofter et al.
Interference of sex-hormone binding globulin in a no-extraction double-antibody radioimmunoassay for estradiol.
Radioimmunoassay of testosterone-estradiol-binding globulin in humans: a reassessment of normal values.
By combining our new FDA-cleared immunoassays, which are equivalent in effectiveness and sensitivity to radioimmunoassay, with our new patented method of releasing the drugs from the hair, we continue to offer truly proprietary technology that provides superior detection of drugs of abuse.
Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma.
Radioimmunoassay for digoxin with a fully automated continuous flow system.
Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay.
Radioimmunoassay and liquid-chromatographic analysis for free cortisol in urine compared with isotope dilution-mass spectrometry.
Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays.