quantify

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quantify

verb To determine the quantity of; measure
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(1) Since quantification is performed using an overall list of peptides identified from any sample in a study, if possible, it is advantageous to include a sample containing the target protein(s) at a high concentration in order to "seed" the analysis to quantify a maximum number of peptides.
(2) The high mass accuracy and resolution possible with current generation mass spectrometers has dramatically increased the specificity of quantification using MS1 signals.
(3) We provide improved methods to estimate the elution time of a peptide for quantification. Often times there are multiple data-dependent MS2 scans of the same peptide ion within a sample.
We have reported here an MS1 relative quantification method that leverages the high mass accuracy and resolution of modern mass spectrometers, approaches to post-processing individual peptide ion peak areas, and a comparison of this approach to targeted MRMs in four different background matrices.
The only difference is that the model-based approach does not aim at a unique quantification of both the anchor worths and the factor importances, but instead focuses on an explicit definition of the scaling and weighting properties.
Finally, the evaluation expert led a discussion session focusing on the quantification principles which could be derived from the different proposals.
The contribution is significant because the framework allows an estimation of the extent of systematic bias related to the quantification and weighting stages of the point method of job evaluation.
One alternative approach then consists of proposing a less general formulation of the quantification principles.
Coupling of Immunodepletion and Prefractionation to MS-Based Quantification
Although depletion and prefractionation alone showed quantification capability in the microgram per liter range, combination of the 2 enabled penetration into the nanogram per liter range, an approximate 1000-fold increase compared with the milligram per liter detection in untreated plasma (41).
In a subsequent study, the same group used a combination of 12 protein depletions with minimal fractionation by strong cation exchange for the multiplexed quantification in the low microgram per liter range of 6 proteins relevant to cardiac injury (42).
Depletion and/or fractionation can improve detection, and, as exemplified by the study mentioned above, allow for quantification in clinically relevant concentrations for some proteins.

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