enhancement for better detection limit and sequencing homopolymers," Biochemical and Biophysical Research Communications, vol.
A total of 302,327 valid sequences of 16 samples were obtained after 454 pyrosequencing
and 158,015 high quality sequences after filtering and removing chimera, in which the most abundant sequences were obtained from the sample DQ, and the least were from the sample CY, as shown in Table 1.
DNA extraction, 454 Pyrosequencing
and Sequence Analysis
Table 1--Detection of LAM resistance mutations by direct sequencing and pyrosequencing
. Source LAM resistance M1 2429 1181 mutations detection HBV-RNA Direct YMDD YMDD YVDD sequencing Pyrosequencing
YMDD (94%) YMDD YMDD (30%) YIDD (6%) YVDD (70%) HBV-DNA Direct YMDD YMDD YVDD sequencing Pyrosequencing
YMDD (96%) YMDD YVDD YIDD (4%)
The methylation patterns of CDKN2A, FOXA1, HOXD8, OCT4, SOX2, and SOX17 were evaluated by pyrosequencing
. As shown in Figure 1, no significant differences were observed when all tested patient groups were evaluated.
. Initial pyrosequencing
PCR assays with the validation primers described above for B.
This has implications for barcoding pyrosequencing
studies which cover high sample numbers, because less barcoded PCR amplicons per sample will be detected and, therefore, dominant bacteria will play a larger role in determining the bacterial composition.
A total of 299 ant samples were subjected to 454 pyrosequencing
and combined with data from 11 samples analyzed by Ishak et al.
The aim of this study was to describe the fungal communities present in fecal samples obtained from healthy dogs and dogs with acute, nonhemorrhagic diarrhea using high-throughput 18S rRNA gene pyrosequencing
DNA was then subjected to 454 pyrosequencing
, a "fingerprinting" technique that provides a snapshot of all the bacterial types present.
We also discuss the limitations of the Sanger DNA sequencing technique in quality control testing of pDNA and suggest using the more sensitive DNA pyrosequencing
, a rapid sequencing method that can simultaneously identify microbes and detect antibiotic resistance, has been applied for the determination of bacterial diversity in chronic wound biofilms such as in diabetic foot ulcers, venous leg ulcers and pressure ulcers.