The role of membrane glycoprotein plasma cell antigen 1/ectonucleotide pyrophosphatase
phosphodiesterase 1 in the pathogenesis of insulin resistance and related abnormalities.
Activity assays showed that, like LpxH, LpxG is a [Mn.sup.2+]-dependent pyrophosphatase
that catalyzes the nucleophilic attack of water on the [alpha]-phosphate of UDPDAG [49, 53].
Goldman, "Inorganic pyrophosphatases
: one substrate, three mechanisms," FEBS Letters, vol.
Liu et al., "Electrochemical strategy for pyrophosphatase
detection Based on the peroxidase-like activity of G-quadruplex-[Cu.sup.2+] DNAzyme," Talanta, vol.
The aqueous layer was collected and a reagent (0.44 U/ml nucleotide pyrophosphatase
, 0.0625 U/ml NADase in 2.5 mmol/L MgCl[sub]2, 12.5 U/ml alkaline phosphatase, and 20 mmol/L sodium phosphate pH 8.0) was added and incubated overnight at 37[degrees]C.
Molecular cloning and characterization of a vacuolar H+ pyrophosphatase
gene, SSVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of arabidopsis.
(40 U/ml) and Sequenase version 2.0 T7 DnA polymerase (13 U/ml) were obtained from USB Corporation (Cleveland, OH, USA).
Woolf, "Effect of hot water treatments on chilling injury and heat damage in 'satsuma' mandarins: antioxidant enzymes and vacuolar ATPase, and pyrophosphatase
," Postharvest Biology and Technology, vol.
Terkeltaub, Up-Regulated Expression of the Phosphodiesterase Nucleotide Pyrophosphatase
Family Member PC-1 is a Marker and Pathogenic Factor for Knee Meniscal Cartilage Matrix Calcification, Arthritis Rheum.
(3) Magnesium is known to be one of the cofactors of many pyrophosphatases
and there is a direct relationship between the solubility of calcium pyrophosphatase
and serum concentration of magnesium.
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A functional aquaporin co-localizes with the vacuolar proton pyrophosphatase
to acidocalcisomes and the contractile vacuole complex of Trypanosoma cruzi.