This is clearly illustrated by the structure determination of pyrogallol
HMTA (1/1), in which despite the presence of two entirely different molecular components in the structure, and the evidence of preferred orientation in the data, structure solution and refinement ran smoothly.
Gallic acid, pyrogallol
, homogentisic acid, protocatechuic acid, (+) catechin, chlorogenic acid, caffeic acid, vanillin, ferulic acid, naringin, resveratrol, naringenin, hesperetin, formononetin and biochanin-A were used as standard for the detection of phenolic compounds from the fruiting bodies of P.
The activity of the isolated laccase toward other substrates including ABTS, N,N-dimethyl-1,4-phenylenediamine, hydroquinone, catechol, 2-methylcatechol, pyrogallol
, and tyrosine was also tested.
The phenolic compounds, found in water extract of green parts of Glycine hispida, are pyrogallol
and phenolic acids.
We investigated the effect of hemolysis on Pyrogallol
Red (PYR) (2), benzethonium chloride (BTC) (3), and benzalkonium chloride (BC) (4) methods for measurement of protein.
More detailed structure-activity investigations are invited to address the interplay of galloyl and pyrogallol
moieties, as well as further studies on structural and stereochemical prerequisites for cannabinoid signaling.
REACTIVITY OF DIFFERENT PROTEINS AND POLYPEPTIDES IN ENDPOINT AND KINETIC BIURET METHODS AND A PYROGALLOL
The determination of superoxide dismutase (SOD) activity was based on the inhibition of pyrogallol
autooxidation (Misra and Fridovich 1972).
2001), porcine retinal homogenate oxidation accelerated by ferric ion (Hiramitsu and Armstrong, 1991), pyrogallol
autaoxidation (Kim and Godber, 2001) and pharmaceutical oils (Juliano et al.
is produced as one of the metabolite of gallic acid, but in this study pyrogallol
was also not detected.
Involvement of the superoxide anion radical in the auto-oxidation of pyrogallol
and convenient assay for superoxide dismutase.
Total SOD activity was assayed as the inhibition of the autooxidation of pyrogallol
in alkaline solution monitored at 420 nm (20), with 1 U of activity giving 50% inhibition at 10 min.