Hyperhomocysteinemia and low
pyridoxal phosphate. Common and independent reversible risk factors for coronary artery disease.
Pyridoxal Phosphate Inhibits Pituitary Cell Proliferation And Hormone Secretion.
Alanine aminotransferase kit {(International Federation of Clinical Chemistry (IFCC)} without
pyridoxal phosphate activation.
Leklem and Shultz (18) indicate that exercise stimulates the release and activation of the enzyme glycogen phosphorylase
pyridoxal phosphate. Thus, the increase in plasma
pyridoxal phosphate seen with exercise may be due to this stimulation of muscle reserves.
Plasma vitamin B6 (pyridoxal and
pyridoxal phosphate) concentration was measured by high-performance liquid chromatography (Thermo Finnigan Spectra Systems HPLC, USA).
Secondly, alcohol consumption causes lowering of the plasma concentration of
pyridoxal phosphate. As shown in the pathway of haem synthesis (Figure 1),
pyridoxal phosphate is a cofactor for ALAS.
Homogenates were sedimented at 26,000 x g for 10 min to remove storage vesicles containing catecholamines, which interfere with TH activity, and assays were conducted with 100 [micro]L aliquots of the supernatant solution in a total volume of 550 [micro]L Each assay contained final concentrations of 910 [micro]M FeS[O.sub.4], 55 [micro]M unlabeled L-tyrosine (Sigma), 9.1 [micro]M
pyridoxal phosphate (Sigma), 36 [micro]M [beta]-mercaptoethanol, and 180 [micro]M 2-amino-6,7-dimethyl-4-hydroxy5,6,7,8-tetrahydropteridine HCl (Sigma), all in a buffer of 180 mM sodium acetate and 1.8 mM sodium phosphate (pH 6.1).