Elimban, "Mechanisms of the beneficial effects of vitamin B6 and pyridoxal 5-phosphate
on cardiac performance in ischemic heart disease," Clinical Chemistry and Laboratory Medicine, vol.
To be biologically active, pyridoxamine is first converted by the body to pyridoxal 5-phosphate (PLP).
* Pyridoxamine is a specialized form of vitamin B6 easily converted in the body to pyridoxal 5-phosphate (PLP), the active form of the vitamin.
VITAMINS [B.sub.1], [B.sub.2], AND [B.sub.6] Vitamin [B.sub.1], [B.sub.2], and [B.sub.6] status was determined by direct measurement of thiamin diphosphate (TDP), FAD, and pyridoxal 5-phosphate
(PLP) in plasma, whole blood, or erythrocytes (1, 3, 15,16).
Homogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate
. Clin Chem 2002;48: 1560-4.
The chemicals used were L-cysteine, D,L-homocysteine, L-methionine, adenosine (ADO), L-dithiothreitol, Triton X-100, pyridoxal 5-phosphate
, and potassium ferricyanide and were purchased from Sigma Chemical Co.
The rHCYase reaction (0.05 g/L in 40 mmol/L sodium phosphate buffer, pH 8.3, containing 20 [micro]mol/L pyridoxal 5-phosphate
) was then run for 3.5 min.
Pyridoxine reduces cholesterol and low-density lipoprotein and increases antithrombin III activity in 80-year-old men with low plasma pyridoxal 5-phosphate
. Scand J Clin Lab Invest 1990;50: 873-7.
at a concentration of 0.1 mmol/L was sufficient.
Vitamin B6 was measured as its first active metabolite, pyridoxal 5-phosphate
, by an enzymatic method .