In this study, the cell packaging density of pyramidal cell layer
(PCL) of CA subfields and granular cell layer (GCL) of the dentate gyrus were examined and compared in the three groups (CTM treated, saline and non-treated) using the Nissl staining technique.
Analysis of neuron cell bodies in the pyramidal cell layer
of CA-1 region of hippocampus was performed.
Immunohistochemistry of brain sections revealed that DSCAM is highly expressed in the cerebral cortex, granule layer of the dentate gyrus, pyramidal cell layer
of Ammon's horn of the hippocampus (stratum pyramidale), thalamus, and cerebellar Purkinje cells of both WT (Figure 2A,C,E) and APP transgenic mice (Figure 2B,D,F).
Mossy fiber axons were electrically stimulated with a bipolar electrode (glass pipette with tip diameter of ~4 [micro]m filled with media solution, glued to a fine tungsten rod) placed in stratum lucidum (30-50 [micro]m from the edge of the pyramidal cell layer
) at a lateral distance of 75-200 [micro]m from the recording pipette.
Prion diseased animals showed microglial invasion of pyramidal cell layer. Viral infection and prion disease acting together cause a significant increase in the microgliogenesis (Tables 3-5).
At 17th wpi prion disease also induced significant microgliosis on pyramidal cell layer and stratum radiatum (two-tail /-tests for independent samples, p < 0.05).
As can be seen in the representative images in the panel, treatment with [A[beta].sub.(1-42)] leads to a discrete but significant reduction ([F.sub.(5), (29)] = 4.241,P< 0.05) in the width of the pyramidal cell layer
in the control groups, a degenerative change absent in the POEE-treated group.
Axon arborizations were rarely observed in the pyramidal cell layer
. Slow voltage ramps (-130 to 20 mV) revealed that the outward current induced by NE reversed at the predicted equilibrium potential for potassium ([K.sup.+]) ions and showed prominent inward rectification, suggesting that NE activates G protein-coupled inwardly rectifying [K.sup.+] channels (GIRK) channels in these interneurons.
The Cavalieri method was used to estimate the total volume of the dentate gyrus and the pyramidal cell layer of the hippocampus.
Two-way ANOVA indicated no significant effect on diet [F(1,28)=1.77;p=0.19] and sex [F(1,28)=0.01; p=0.98]; but a significant dietXsex interaction [F(1,28)=5.54; p<0.05] in the volumetric estimation of the pyramidal cell layer.
The number of cells positive for BDNF, TrkB and p75NTR was assessed in the granular cell layer of the DG and the pyramidal cell layer
of the CA1, CA3 and CA4 regions.
One way-ANOVA statistical test was applied to analyze the volume and numerical density of hippocampal pyramidal cell layer
. P-value of less than 0.05 was considered as significant at 95% confidence interval.