Then 100 [micro]L of bacterial suspension was spread by glass rod, 20 [micro]L of purified pyocyanin
was added to the prepared wells in the same plate and then incubated at 37[degrees]C for 24 h and the diameter of zone was measured and the results were recorded.
(32) The prevalences of the virulence genes algD, lasB, toxA and secretion of pyocyanin
in this study were very high among P.
Winstanley, "Widespread pyocyanin
overproduction among isolates of a cystic fibrosis epidemic strain," BMC Microbiology, vol.
Cohen, "Response of Pseudomonas aeruginosa to pyocyanin
mechanisms of resistance, antioxidant defenses, and demonstration of a manganese-cofactored superoxide-dismutase," Infection and Immunity, vol.
has antibiotic activity against a wide variety of microorganisms (Liang et al., 2008).
The overexpression of PvdQ in P aeruginosa strains is shown to inhibit the accumulation of signal molecules (3-oxo-C12-HSL and 2-heptyl-3-hydroxy-4(1H)-quinolone) and thereby decreases expression of several virulence factors such as elastase and pyocyanin
that are important for P aeruginosa pathogenicity in animals .
mallei produce toxins such as pyocyanin
(blue-green pigment that interferes with energy production via the electron transfer system), lecithinase (causes cell lysis by degrading lecithin of cell membranes), collagenase, lipase, and hemolysin.
Main characteristics of selected isolates Metabolite/Isolatecode AJ B 2 AJ D 1 AJ D 2 Indole Acetic Acid([micro]g/ml) 44 62 70 PhosphateSolubilisation (mg/lit) ND ND ND Chitinase (IU) ND ND 170 Bacitracin ([micro]g/ml) ND 11 7 Pyocyanin
([micro]g/ml) ND ND NS Siderophore production(in %) ND 69.33 ND Silver nanoparticles Y ND ND ND = Not detect NS = Not significant Metabolite/Isolatecode AJ D 3 AJ E 3 AJ A 2 Indole Acetic Acid([micro]g/ml) 70 25 38 PhosphateSolubilisation (mg/lit) ND ND 13.25 Chitinase (IU) 710 870 ND Bacitracin ([micro]g/ml) 6 ND ND Pyocyanin
([micro]g/ml) 3.15 ND ND Siderophore production(in %) ND 68.83 ND Silver nanoparticles Y ND ND ND = Not detect Y = Able to produce Table 5.
Biochemical test seudomonas aeruginosa Catalase test (+)ve Oxidase test (+)ve simmon citrate test (+)ve Urease test (-)ve TSI (-)ve Indol test (-)ve Motility test (+)ve Methyl red test (-)ve Vogas-proskaure test (-)ve Gram stain (-)ve Cell morphology Pseudomonas aeruginosa growth on tryptic soy agar and Cultivation for 24 hours at 37[degrees]C +; then at room temperature the Basic shape of colonies were circular; Elevated and Pigmet production like pyocyanin
. Table 3: Results of sensitivity test of, Klebsiella pneumonia isolates against 7 of antibiotics.
Pseudomonas aeruginosa species were identified by Gram staining motility catalase oxidase and pyocyanin
production tests while identification and confirmation of Staphylococcus aureus strains were done by colonial morphology on blood agar Gram stain haemolysis catalase and coagulase tests.1112
aeruginosa, based on the colony morphology, gram staining, oxidase reaction, production of the pyocyanin
pigment, nitrate reduction, use of citrate and malonate as carbon sources, and their ability to grow at 5 [degrees]C and 42 [degrees]C.
Cultures revealed Pseudomonas aeruginosa which is a gram-negative aerobic bacillus that produces the pigments pyocyanin
and pyoverdin that responsible for this characteristic hue.