puromycin


Also found in: Dictionary, Encyclopedia, Wikipedia.

puromycin

(pyo͝or′ə-mī′sĭn)
n.
An antibiotic, C22H29N7O5, obtained from the soil bacterium Streptomyces alboniger, that is used experimentally as an inhibitor of protein synthesis.

puromycin

Puromycin aminonucleoside Experimental biology An antibiotic that inhibits protein synthesis isolated from Streptomyces alboniger, used to induce experimental lipoid nephrosis in animals
References in periodicals archive ?
(c) Transduced hAD-MSCs after puromycin selection; abundant green cells and GFP expression exhibit high transduction level (d) FACS analysis of the transduction rate after puromycin selection indicated that more than 90% of the cells were GFP positive.
The recombinant plasmid was then transfected into porcine IPEC-J2 cells, followed by 2-week puromycin selection to achieve stable integration of the luciferase reporter gene.
From above, cells were co-transfected with circular TALENs and linearized pBLC-TK, plated on 6-well plates and maintained at 37[degrees]C, under puromycin selection.
Because the lentivirus vector contains both GFP gene and puromycin resistance gene, we used both markers for evaluation and selection of transductants.
Studies have shown that MtD may be involved in the development of FSGS in humans and may play a role in puromycin and aldosterone-induced renal injury [3, 19].
Then, puromycin selection was performed in the presence of 2 mg/ml puromycin for 2 weeks.
After 48 h, Hygromycin B (500 [micro]g/mL; Sigma-Aldrich), Neomycin (750 [micro]g/mL; Life Technologies), and Puromycin (5 [micro]g/mL; Sigma-Aldrich) were added to cell cultures.
The strand also has a puromycin selection gene for enriching cell populations that uptake the RNA strand, increasing reprograming efficiencies and success.
The [hUDSCs.sup.miR-375+] were exposed to 2.5 [micro]g/mL puromycin for 3days to obtain stable transduction.
After lentivirus infection and puromycin selection, the drug-resistant cells were obtained.