A, Screening of monoclonal cells by puromycin
(c) Transduced hAD-MSCs after puromycin
selection; abundant green cells and GFP expression exhibit high transduction level (d) FACS analysis of the transduction rate after puromycin
selection indicated that more than 90% of the cells were GFP positive.
The recombinant plasmid was then transfected into porcine IPEC-J2 cells, followed by 2-week puromycin
selection to achieve stable integration of the luciferase reporter gene.
From above, cells were co-transfected with circular TALENs and linearized pBLC-TK, plated on 6-well plates and maintained at 37[degrees]C, under puromycin
Because the lentivirus vector contains both GFP gene and puromycin
resistance gene, we used both markers for evaluation and selection of transductants.
Studies have shown that MtD may be involved in the development of FSGS in humans and may play a role in puromycin
and aldosterone-induced renal injury [3, 19].
selection was performed in the presence of 2 mg/ml puromycin
for 2 weeks.
Infected cells were selected and continously maintained in the presence of 1.5 [micro]g/mL puromycin
After 48 h, Hygromycin B (500 [micro]g/mL; Sigma-Aldrich), Neomycin (750 [micro]g/mL; Life Technologies), and Puromycin
(5 [micro]g/mL; Sigma-Aldrich) were added to cell cultures.
The strand also has a puromycin
selection gene for enriching cell populations that uptake the RNA strand, increasing reprograming efficiencies and success.
The [hUDSCs.sup.miR-375+] were exposed to 2.5 [micro]g/mL puromycin
for 3days to obtain stable transduction.
After lentivirus infection and puromycin
selection, the drug-resistant cells were obtained.