selection, the cells were identified using real-time PCR and Western blotting.
For agarose gel detection, either CHX (cydoheximide) or puromycin
Four kinds of plasmid, U6, K1, K2 and K3, were constructed, introduced into C2C12 myocytes, and the transformed cells were selected using puromycin
To apply the cumate-inducible transgene expression system, the MyoD gene, an important regulator of muscle differentiation, was inserted into the piggyBac cumate-inducible expression vector, and stably transfected DF1 cells were established following transfection and puromycin
mice and treated with apoptosis inducers cycloheximide (CHX) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL) and puromycin
(PM) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL), respectively, to validate their inability to undergo apoptosis.
Mitochondrial dysfunction in focal segmental glomerulosclerosis of puromycin
Successfully transfected HepG2 cells were then selected by puromycin
(1 mg/L) for 2 weeks.
The individual stably transfected colony was subsequently selected in the presence of puromycin
To address the hypothesis that AHR activation by TCDD during embryonic development disrupts expression of genes critical to cardiac differentiation, we generated an AHR-positive embryonic stem cell lineage that expresses puromycin
resistance and enhanced green fluorescent protein (eGFP) under the control of the AHR-responsive Cyplal promoter.
The cells carrying the transgene were selected in medium containing 1 mg/ml puromycin
(Gibco BRL) for 5 days.
To obtain stable-transfected cells lines, cells were subcultured with 5 ug/ml puromycin
for 2 weeks.
After ADSCs were exposed to lentivirus for 24 h, medium was replaced with fresh complete medium containing 2% puromycin
for a further 24 h to select for transfected cells.