Meanwhile, rapidly fermentable energy and protein sources showed higher DM digestibility, VFA production, ammonia-N utilization, and
purine base contents, indicating that the availability of energy or the protein source should be also considered as the other important factor for improving rumen fermentation and MPS.
The following peculiarity was observed by application surfactant solutions as eluents for
purine bases separation: the chromatographic chamber did not require long-time pretreatment for saturation by solvent vapor.
In a mixture of nitrogenated bases, the oxidation peaks of the
purine bases are shifted towards less positive potentials in relation to their pure solutions.
Contribution of dietary nitrogen and
purine bases to the duodenal digesta: comparison of duodenal and polyesterbag measurements.
Because
purine bases and cytosine are found in both RNA and DNA, the concentrations of purine and cytosine are higher than that of uracil [17].
Comparison of microbial markers ([sup.15.N] and
purine bases) and bacterial isolates for the estimation of rumen microbial protein synthesis.
Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of 15N and
purine bases as microbial markers: effect of protein source and rumen bacterial isolates.
As described earlier, creatinine excretion is usually independent of the level of intake or duodenal infusion of
purine bases (IAEA, 2002).
The
purine bases of feces were analysed according to Martinez-Puig et al [20], with high performance liquid chromatography specifications as described by Vlaeminck et al [21].