pulsed-field gel electrophoresis


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pulse-field gel e·lec·tro·pho·re·sis

gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules.

pulsed-field gel electrophoresis (PFGE)

GEL ELECTROPHORESIS in which the gel is subjected to electric fields alternating between different angles. This allows large DNA MOLECULES, such as complete chromosomes, to become reorientated with the change in electric field, so that they can migrate, in a snake-like manner, through the gel for separation.
References in periodicals archive ?
Typing of group B streptococci: comparison of pulsed-field gel electrophoresis and conventional electrophoresis.
Analysis of Bordetella pertussis isolates from an epidemic by pulsed-field gel electrophoresis. J Clin Microbial 1995;33(12):3083-3086.
Application of pulsed-field gel electrophoresis to molecular epidemiology.
Pulsed-field gel electrophoresis analysis of nasopharyngeal flora in children attending a day care center.
The symptoms were similar to those of reported hot tub-associated cases (often called "hot tub lung"), but multiple samples from the respiratory tract and from the patient's shower and bathtub grew MAC with matching pulsed-field gel electrophoresis patterns, while specimens from his hot tub were negative (Chest 2005; 127:664-71).
Others at California Polytechnic have attempted to classify and identify a collection of probiotic lactobacilli and Bifidobacteria using such phenotypic and gene-based methods as carbohydrate fermentation patterns, fatty acid analysis, pulsed-field gel electrophoresis and 16S rDNA sequencing.
The database comprises demographic and experimental data, including pulsed-field gel electrophoresis (PFGE) fingerprints obtained by using the PulseNet standardized PFGE protocol (3), serotypes obtained using PFGE pattern comparison and conventional methods (4), and antimicrobial drug susceptibility testing results for isolates collected from hospitals nationwide.
Available isolates from patient stool (n = 1), bulk tank milk (n = 1), and retail milk (n = 1) were identified by CDC as Campylobacter jejuni and were indistinguishable by pulsed-field gel electrophoresis (PFGE).
Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil.
Moreover, pulsed-field gel electrophoresis analysis of S infantis isolates revealed a single identical pulsed-field pattern.
These were grouped using three molecular subtyping methods: multilocus sequence typing, pulsed-field gel electrophoresis and ribotyping.

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