Feed restriction resulted in lower levels of antigen-specific IgE in serum and reduced antigenspecific lympho-proliferative activity in pulmonary lymph nodes. Feed restriction also attenuated pulmonary inflammation, as evidenced by lower levels of lactate dehydrogenase and total protein, decreased infiltration of neutrophils and eosinophils, and reduced secretion of pro-inflammatory cytokine tumor necrosis factor (TNF)-[Alpha] in bronchoalveolar lavage fluid.
The spleen and pulmonary lymph nodes were also collected.
The mRNA levels of pro-inflammatory cytokines TNF-[Alpha] and IL-6 in preparations from pooled pulmonary lymph nodes appeared to be lower in the feed-restricted group compared to the ad libitum group 7 days after HDM sensitization (Figure 7).
To investigate the effect of feed restriction on the balance of Th1 and Th2 cytokines, the protein levels of IL-2, IFN[Gamma], IL-4, and IL-10 in serum and in BAL fluid as well as the mRNA levels of IL-2, IFN[Gamma], IL-4, and IL-5 in pulmonary lymph nodes were assessed.
Because the lesions in the mongooses and the suricate were disseminated, the route of infection is not clean However, an oral route of infection is suspected because the pulmonary lesions involved the interstitium and alveolar walls rather than the bronchioles, and mesenteric but not pulmonary lymph nodes
were enlarged (banded mongoose).