The sequence of N2 resembled that of an Arabidopsis thaliana MYB transcription factor, that of N4 a multisubstrate pseudouridine
synthase 7-like gene from Malus x domestica, N5 a Gossypium hirsutum putative leucine zipper protein.
Nucleosides, as dimethylguanosine and pseudouridine
are components of RNA that enter the circulation in larger amounts in cases of enhanced cellular proliferation.
applied a GC-MS-based platform to measure 272 metabolite peaks in plasma obtained from 52 individuals with heart failure and 57 age-matched controls, and identified pseudouridine
as the most significant discriminator of disease status (25).
Yu and Karijolich studied pseudouridine
, a slightly different version of the RNA component uridine.
The Role Of Pseudouridine
At The Spliceosomal Branch Site: A Molecular Dynamics Analysis.
The purines (except 2-deoxyadenosine), orotic acid, pseudouridine
, uridine, 2-deoxyuridine, 5-hydroxymethyluracil, and thymidine were ionized in negative ionization mode.
Simultaneous determination of purine metabolites, creatinine and pseudouridine
in ruminant urine by reversed-phase high-performance liquid chromatography.
Automated determination of orotic acid, uracil and pseudouridine
in urine by highperformance liquid chromatography with column switching.
Orotic acid, pseudouridine
, 5-hydroxymethyluracil (5-OH-Me-ura), deoxyinosine, deoxyguanosine, and ammonium formate were purchased from Sigma.
Urine from healthy infants contained dominant peaks of uric acid (UA), urea, creatinine, and hippuric acid and usually various amounts of hypoxanthine (HX), xanthine (X), 7-methylguanine, pseudouridine
, and uridine.
GGT complexed to LDL and VLDL), 5'-nucleotidase, leucine aminopeptidase, cholinesterase, copper, iron, ferritin, and pseudouridine
, using previously described procedures (2).
26(s) Oxypurinol-l-riboside Model compound not available Oxypurinol-7-riboside Model compound not available Pseudouridine