Referring to the nonenzymatic, heat-stable peroxidase activity associated with hemeproteins.
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References in periodicals archive ?
In addition, there has been evidence showing the presence of "pseudoperoxidase" or peroxidase-like activities in hemoglobins [3-8].
The principle of the method for GPx activity measurement was based on recording the decrease in absorbance of NADPH at 340 nm using [H.sub.2][O.sub.2] and reduced glutathione as substrates, in the presence of yeast glutathione reductase and Na[N.sub.3] to inhibit the pseudoperoxidase activity of Hb.
The gFOBT has a long history of use for detecting the presence of blood in the stool and is based on the pseudoperoxidase activity of heme in human hemoglobin.
[H.sub.2][O.sub.2] is toxic to the cells and recently it has been shown that Hb also possesses antioxidative pseudoperoxidase activity.
This method detects the pseudoperoxidase activity of hemoglobin; thus, endogenous salivary peroxidases produce false-positive results for hemoglobin.
Ascorbic acid as an antioxidant may yield false negative results when it interferes with the pseudoperoxidase reaction[12].
This procedure inhibited endogenous AP, peroxidase, and pseudoperoxidase activity present in tissues.
It is also relevant to mention that above-therapeutic concentrations of mesna, which carry a high reducing capacity, did not cause interference in two Chemstrip 10UA tests that are based on oxidation reactions (glucose by glucose oxidase/peroxidase and hemoglobin with its strong pseudoperoxidase activity).
A second obstacle was the pseudoperoxidase activity of hemoglobin, which can catalyze side reactions with hydrogen peroxide.
A microhematuria/hemoglobinuria assay performed at the same time utilizes the pseudoperoxidase activity of hemoglobin.