Liquid chromatography-mass spectrometry analysis of HPL extract showed that it contains over two dozens of constituents, among which hyperforin, hypericin, pseudohypericin
, quercetin, chlorgenic acid, rutin, hyperoside, amentoflavone, etc.
During a bio-assay guided fractionation of a methanolic extract, hypericin and pseudohypericin
were identified as components that showed pharmacological effect in the forced swimming test while the purified compounds were found to be inactive after treatment at doses comparable to the total extracts.
(10 [micro]M) reduced NMDAinduced ion currents by 20% and GABA-induced chloride currents by 57%.
The anti-inflammatory effect of Hp is attributed by Bezakova et al (1999) to its isolated constitutes anthrone, anthroquinone, emodin hypericin and pseudohypericin
while its analgesic effect may be attributed to the presence of flavonoids, tannins and saponins (Sanchez-Mateo 2006, Bezakova 1999).
John's wort (Hypericum triquetrifolium turra), which contains hypericin and pseudohypericin
, both of which show antiretroviral activity in cell cultures.
HPLC (Hewlett Packard Series 1100, Atlanta, GA) analysis was used to identify rutin, hypericin, pseudohypericin
, and hyperforin in the above-mentioned species.
The plant also contains polycyclic phenols, hypericin, and pseudohypericin
, in addition to flavonoids (hyperoside, quercetin, isoquercitrin, rutin), kaempferol, luteolin, and biapigenin.
Gibson says their method takes just 15 minutes to separate hypericin from pseudohypericin
, a related compound, in crude extracts using high-performance liquid chromatography (HPLC).
Hypericin and pseudohypericin
specifically inhibit protein kinase C: possible relation to their antiretroviral activity.
Isolated fractions applied in combinations of procyanidines with naphtodianthrones, hypericin and pseudohypericin
were, however, already significantly active in the FST at much lower concentrations (0.
and some flavonoids were identified or tentatively identified.
Hypericin plus pseudohypericin
content, determined by high-performance liquid chromatography (HPLC) and detected by diode array detection, was specified as minimally 50% of the determined total hypericin content.