Chemical constituents: Volatile oil, hypericin, and pseudohypericin
, flavonoids (Khare, 2007; Prajapati et al.
The most important active ingredients of hypericum are hypericin and pseudohypericin
Liquid chromatography-mass spectrometry analysis of HPL extract showed that it contains over two dozens of constituents, among which hyperforin, hypericin, pseudohypericin
, quercetin, chlorgenic acid, rutin, hyperoside, amentoflavone, etc.
During a bio-assay guided fractionation of a methanolic extract, hypericin and pseudohypericin
were identified as components that showed pharmacological effect in the forced swimming test while the purified compounds were found to be inactive after treatment at doses comparable to the total extracts.
(10 [micro]M) reduced NMDAinduced ion currents by 20% and GABA-induced chloride currents by 57%.
The anti-inflammatory effect of Hp is attributed by Bezakova et al (1999) to its isolated constitutes anthrone, anthroquinone, emodin hypericin and pseudohypericin
while its analgesic effect may be attributed to the presence of flavonoids, tannins and saponins (Sanchez-Mateo 2006, Bezakova 1999).
perforatum reported increases in hypericin and pseudohypericin
levels in shoots grown on BA-containing medium, suggesting that stimulation of hypericin production by BA is probably due to the increase in the number of dark glands in the leaves of the in vitro shoots (GADZOVSKA et al.
John's wort (Hypericum triquetrifolium turra), which contains hypericin and pseudohypericin
, both of which show antiretroviral activity in cell cultures.
1995) and a great number of studies refer to the biologically active components isolated: hypericin, pseudohypericin
, hyperforin, adhyperforin, procyanidins andxanthones.
The major constituents of the herb include: hypericin, hyperforin, pseudohypericin
, flavonoids, xanthones, and essential oils.
The samples were then diluted four times before pseudohypericin
and hypericin concentration determination.
HPLC (Hewlett Packard Series 1100, Atlanta, GA) analysis was used to identify rutin, hypericin, pseudohypericin
, and hyperforin in the above-mentioned species.